JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 30: Issue 2
Separation and detection of bioactive essential oil components by overpressured layer chromatography coupled with bioactivity tests
Authors: Ágnes M. Móricz 1 , Dániel Krüzselyi 1 , and Péter G. Ott 1
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  • 1 Hungarian Academy of Sciences, 15, Herman Ottó Street, H-1022 Budapest, Hungary
DOI:
https://doi.org/10.1556/1006.2017.30.2.5
Page Count:
121–125
Publication Date:
01 Apr 2017
Online Publication Date:
Apr 2017
Article Category:
Research Article
Restricted access

Overpressured layer chromatography (OPLC), ensuring pumpforced constant mobile phase flow and the possibility of overrun, offers the expanded exploitation of fine-particle adsorbent layers for a longer development distance. Using an infusion—transfusion OPLC method with a 26-cm long development, the separation of clove, rosemary, eucalyptus, tea tree, spearmint, thyme, and cinnamon bark essential oil components was achieved with good resolutions. In the combination of OPLC and Aliivibrio fischeri assay, the main essential oil components eugenol, borneol, (−)-R-carvone, thymol, and trans-cinnamaldehyde exhibited antibacterial effect. The OPLC—2,2-diphenyl-1-picrylhydrazyl (DPPH*) test showed two antioxidant components: eugenol and thymol.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993
Keywords:
Overpressured layer chromatography; High-performance thin-layer chromatography; Direct bioautography; Luminescent Aliivibrio fischeri; Antioxidant 2,2-diphenyl-1-picrylhydrazyl test; Essential oils

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