JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 30: Issue 2
Comparison of the microbiological activities of different varieties of hop (Humulus lupulus) extracts by thin-layer chromatography—direct bioautography
Authors:
Grzegorz Jóźwiak Medical University of Lublin, 4a, Chodźki Street, 20-093 Lublin, Poland

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Barbara Majer-Dziedzic University of Life Sciences, Akademicka Street, 20-950 Lublin, Poland

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Justyna Kwiecińska Medical University of Lublin, 4a, Chodźki Street, 20-093 Lublin, Poland

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Monika Waksmundzka-Hajnos Medical University of Lublin, 4a, Chodźki Street, 20-093 Lublin, Poland

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Pages:
126–130
Online Publication Date:
Apr 2017
Publication Date:
01 Apr 2017
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2017.30.2.6
Keywords:
Hop (Humulus lupulus) varieties; Thin-layer chromatography; Bioautography
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Commercially available hop pellets of different origins were extracted by use of ethanol and water, chromatographed on silica layers by use of nonaqueous eluents, chemically derivatized and observed in ultraviolet (UV) light for the localization of component bands. The plates were developed in optimized systems, and direct bioautographic method by use of Bacillus subtilis and Escherichia coli strains was applied for the examination of the antimicrobial activities of hop components. The method enables for the identification of bactericidal/bacteriostatic components in the extracts of different polarities and shows differences in the composition of extracts from various varieties from an antimicrobial point of view.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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