JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 30: Issue 4
High-performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl
Authors:
Nadja Meier Zurich University of Applied Sciences, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland

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,
Beat Meier Zurich University of Applied Sciences, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland

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Samuel Peter Zurich University of Applied Sciences, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland

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Evelyn Wolfram Zurich University of Applied Sciences, Einsiedlerstrasse 31, 8820 Wädenswil, Switzerland

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Pages:
238–244
Online Publication Date:
Aug 2017
Publication Date:
01 Aug 2017
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2017.30.4.1
Keywords:
High-performance thin-layer chromatography; Senna; Cassia senna L.; Cassia angustifolia Vahl; Sennosides; Identity testing Ph. Eur.
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A Ph. Eur. herbal drug monograph requires for identification testing besides macroscopic (A) and microscopic (B) evaluation a thin-layer chromatography (TLC) fingerprint, prescribed as identification testing in subsection C. The main active constituents of Cassia senna L. and Cassia angustifolia Vahl are the dianthrone glucosides (mainly sennoside A and B). Therefore, this study presents a high-performance thin-layer chromatography (HPTLC) method which uses the sennosides as characteristic constituents. The presented method results in a fast and simple fingerprint with characteristic yellow fluorescence for the sennosides due to derivatization with a potassium hydroxide solution. The use of a senna herbal drug dry extract as system suitability test (SST) allows for a cost-effective test control. The resolution of the sennosides zones is dependent on the extractives concentration. An overload of the analytes leads to tailing (u-shaped form) and broadening as well as change in the retardation factor (RF). The presented method is a suitable fingerprint method for Ph. Eur. conform identification C.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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