JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 31: Issue 1
Effect-directed analysis of Pimpinella saxifraga L. root extract via HPTLC–UV/Vis/FLD–EDA–MS
Authors:
Gertrud E. Morlock Justus Liebig University Giessen, Heinrich-Buff-Ring 26–32, 35392 Giessen, Germany

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and
Tatjana Lapin Justus Liebig University Giessen, Heinrich-Buff-Ring 26–32, 35392 Giessen, Germany

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Pages:
79–86
Online Publication Date:
Feb 2018
Publication Date:
01 Feb 2018
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2018.31.1.10
Keywords:
Polyphenols; Antimicrobials; Acetylcholinesterase inhibitors; Bioactive compounds; Planar yeast estrogen screen; Direct bioautography; Planar chromatography-bioassays
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A method was developed for effect-directed analysis (EDA) of the root extract of Pimpinella saxifraga L. High-performance thin-layer chromatography (HPTLC) was hyphenated with microchemical, biochemical, and biological assays as well as electrospray ionization– mass spectrometry (ESI–MS). This HPTLC–UV/Vis/FLD– EDA–MS method directly pointed to multi-potent compounds in the P. saxifraga L. root extract. 2,2-Diphenyl-1-picrylhydrazyl radical scavengers, acetylcholinesterase inhibitors, estrogen-effective compounds, antimicrobials against Gram-positive Bacillus subtilis bacteria, and Gram-negative Aliivibrio fischeri bacteria were discovered in the root extract. A first targeted characterization of four unknown multi-potent compounds was performed by HPTLC–ESI–MS and microchemical derivatizations. This highly streamlined effect-directed profiling is recommended for a fast and cost-efficient natural product search.

  • [1]

    N. Tabanca , E. Bedir, N. Kirimer, K.H.C. Baser, S.I. Khan, M.R. Jacob, I.A. Khan, Planta Med. 69 (2003) 933–938.

  • [2]

    N. Tabanca , S.I. Khan, E. Bedir, S. Annavarapu, K. Willett, I.A. Khan, N. Kirimer, K.H.C. Baser, Planta Med. 70 (2004) 728–735.

  • [3]

    N. Tabanca , A.W. Douglas, E. Bedir, F.E. Dayan, N. Kirimer, K.H.C. Baser, Z. Aytac, I.A. Khan, P.E. Scheffler, Plant Genet. Resour. 3 (2) (2005) 149–169.

    • Search Google Scholar
    • Export Citation
  • [4]

    N. Tabanca , E. Bedir, D. Ferraaira, D. Slade, D.E. Wedge, M.R. Jacob, S.I. Khan, N. Kirimer, K.H.C. Baser, I.A. Khan, Chem. Biodivers. 2 (2005) 221–232.

    • Search Google Scholar
    • Export Citation
  • [5]

    N. Tabanca , B. Demirci, N. Kirimer, K.H.C. Baser, E. Bedir, I.A. Khan, D.E. Wedge, J. Chromatogr. A 1097 (2005) 192–198.

  • [6]

    N. Tabanca , B. Demirci, T. Ozek, N. Kirimer, K.H.C. Baser, E. Bedir, I.A. Khan, D.E. Wedge, J. Chromatogr. A 1117 (2006) 194–205.

  • [7]

    B. Tepe , H.A. Akpulat, M. Sokmen, D. Daferera, O. Yumrutas, E. Aydin, M. Polissiou, A. Sokmen, Food Chem. 97 (2006) 719–724.

  • [8]

    K. Skalicka-Wozniak , M. Walasek, A. Ludwiczuk, K. Głowniak, J. Sep. Sci. 36 (2013) 2611–2614.

  • [9]

    N. Andarwulan , K. Shetty, Food Biotechnol. 14 (2000) 1–20.

  • [10]

    R. Kleiman , D.R. Plattner, D. Weisleder, J. Nat. Prod. 52 (1988) 249–256.

  • [11]

    C. Marcus , E.P. Lichtenstein, J. Agric. Food Chem. 27 (1979) 1217–1223.

  • [12]

    J. Reichling , B. Merkel, J. Nat. Prod. 54 (1991) 1416–1418.

  • [13]

    J. Reichling , R. Martin, B. Kemmeter, Plant Cell Tiss. Org. 43 (1995) 131–136.

  • [14]

    A. Grys , E. Komorowska, S. Mielcarek, Z. Lowicki, K. Latowski, W. Buchwald, Herba Pol. 55 (2009) 109–117.

  • [15]

    F. Wessely , L. Neugebauer, Monatsh. Chem. 84 (1953) 217.

  • [16]

    A.A.F. Zielinski , C.W.I. Haminiuk, A. Alberti, A. Nogueira, I.M. Demiate, D. Granato, Food Res. Int. 60 (2014) 246–254.

  • [17]

    Y. Zuo , H. Chen, Y. Deng, Talanta 57 (2002) 307–316.

  • [18]

    T. Ishikawa , E. Fujimatu, J. Kitajima, Chem. Pharm. Bull. 50 (2002) 1460–1466.

  • [19]

    X. Chang , W. Kang, Med. Chem. Res. 21 (2012) 4324–4329.

  • [20]

    G. Morlock , W. Schwack, J. Chromatogr. A 1217 (2010) 6600–6609.

  • [21]

    G. Morlock , ACS Symp. Ser. 1185 (2013) 101–121.

  • [22]

    Á.M. Móricz , P.G. Ott, T.T. Häbe, A. Darcsi, A. Böszörményi, Á. Alberti, D. Krüzselyi, P. Csontos, S. Béni, G.E. Morlock, Anal. Chem. 88 (2016) 8202–8209.

    • Search Google Scholar
    • Export Citation
  • [23]

    S.S. Teh , G.E. Morlock, Food Chem. 186 (2015) 460–468.

  • [24]

    G. Morlock , W. Schwack, Trends Anal. Chem. 29/10 (2010) 1157–1171.

  • [25]

    G.E. Morlock , in: J. Reedijk (ed.), Elsevier Reference Module in Chemistry, Molecular Sciences and Chemical Engineering, Elsevier, Waltham, MA, USA, 2014.

    • Search Google Scholar
    • Export Citation
  • [26]

    Á.M. Móricz , T.T. Häbe, A. Böszörményi, P.G. Ott, G.E. Morlock, J. Chromatogr. A 1422 (2015) 310–317.

  • [27]

    T.T. Häbe , G.E. Morlock, Rapid Commun. Mass Spectrom. 30 (2016) 321–332.

  • [28]

    Á.M. Móricz , P.G. Ott, G.E. Morlock, in submission.

  • [29]

    S. Lehmann , Master Thesis, Justus Liebig University Giessen, 2014.

  • [30]

    S. Hage , G.E. Morlock, J. Chromatogr. A 1490 (2017) 201–211.

  • [31]

    R. Akkad , W. Schwack, J. Chromatogr. B 878 (2010) 1337–1345.

  • [32]

    S. Krüger , O. Urmann, G.E. Morlock, J. Chromatogr. A 1289 (2013) 105–118.

  • [33]

    International Organization for Standardization, EN ISO 11348-1:2008, Water quality – determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent bacteria test) – part 1: method using freshly prepared bacteria, section 5, Geneva, 2007.

  • [34]

    M. Jamshidi-Aidji , G.E. Morlock, J. Chromatogr. A 1420 (2015) 110–118.

  • [35]

    M. Jamshidi-Aidji , G.E. Morlock, Anal. Chem. 88 (2016) 10979–10986.

  • [36]

    I. Klingelhöfer , G.E. Morlock, J. Chromatogr. A 1360 (2014) 288–295.

  • [37]

    G.E. Morlock , I. Klingelhöfer, Anal. Chem. 86 (2014) 8289–8295.

  • [38]

    T. Nishihara , J. Nishikawa, T. Kanayama, F. Dakeyama, K. Saito, M. Imagawa, S. Takatori, Y. Kitagawa, S. Hori, H. Utsumi, J. Health Sci. 46 (2000) 282–298.

    • Search Google Scholar
    • Export Citation
  • [39]

    A.M. Soto , C. Sonnenschein, K.L. Chung, M.F. Fernandez, N. Olea, F.O. Serrano, Environ. Health Perspect. 103 (1995) 113–122.

  • [40]

    A. Breithofer , K. Graumann, M.S. Scicchitano, S.K. Karathanasis, T.R. Butt, A. Jungbauer, J. Steroid Biochem. Mol. Biol. 67 (1998) 421–429.

    • Search Google Scholar
    • Export Citation
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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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