Authors:
Ágnes M. Móricz Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Street 15, 1022 Budapest, Hungary

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Dániel Krüzselyi Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Street 15, 1022 Budapest, Hungary

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Péter G. Ott Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Street 15, 1022 Budapest, Hungary

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In this study, the antibacterial profiling of the ethanolic leaf extract of greater burdock (Arctium lappa L.) is demonstrated, applying thin-layer chromatography (TLC) coupled bioassays against the Gram-positive soil bacterium Bacillus subtilis and the Gram-negative pepper pathogen Pseudomonas syringae pv. maculicola. The main active component was isolated by eluting from the adsorbent bed and subjected to a targeted characterization by high-performance liquid chromatography–diode array detection–electrospray ionisation–mass spectrometry. The identification of the germacranolide sesquiterpene lactone onopordopicrin was based on its retardation factor, bioactivity in TLC-based methods, and retention tim as well as ultraviolet (UV) and mass spectra, compared to those of the reference substance isolated earlier in our laboratory from Onopordum acanthium leaf.

  • [1]

    A.R.M. Coates , G. Halls, Y. Hu, Br. J. Pharmacol. 163 (2011) 184194.

  • [2]

    P. Järvinen , S. Nybond, L. Marcourt, Q.E. Ferreira, J.L. Wolfender, A. Mettälä, M. Karp, H. Vuorela, P. Vuorela, A. Hatakka, P. Tammela, Pharm. Biol. 54 (2016) 11081115.

    • Search Google Scholar
    • Export Citation
  • [3]

    S. Pata , N. Yaraksa, S. Daduang, Y. Temsiripong, J. Svasti, T. Araki, S. Thammasirirak, Dev. Comp. Immunol. 35 (2011) 545553.

  • [4]

    Á.M. Móricz , P.G. Ott, T.T. Häbe, A. Darcsi, A. Böszörményi, Á. Alberti, D. Krüzselyi, P. Csontos, S. Béni, G.E. Morlock, Anal. Chem. 88 (2016) 82028209.

    • Search Google Scholar
    • Export Citation
  • [5]

    E. Tyihák , Á.M. Móricz, P.G. Ott, in: M. Waksmundzka-Hajnos, J. Sherma, T. Kowalska (eds.), Thin Layer Chromatography in Phytochemistry, CRC Press, Boca Raton, FL, 2008, pp. 193214.

    • Search Google Scholar
    • Export Citation
  • [6]

    G. Morlock , W. Schwack, J. Chromatogr. A 1217 (2010) 66006609.

  • [7]

    I.M. Choma , E.M. Grzelak, J. Chromatogr. A 1218 (2011) 26842691.

  • [8]

    G. Morlock , in: G.K. Jayprakasha, B.S. Patil, F. Pellati (eds.), Instrumental Methods for the Analysis and Identification of Bioactive Molecules, Chapter 5, ACS Symposium Series, vol. 1185, American Chemical Society, Washington, DC, 2014, pp. 101121,

    • Search Google Scholar
    • Export Citation
  • [9]

    Á.M. Móricz , P.G. Ott, J. AOAC Int. 98 (2015) 850856.

  • [10]

    W. Jesionek , B. Majer-Dziedzic, G. Horváth, Á.M. Móricz, I.M. Choma, J. Planar Chromatogr. 30 (2017) 131135.

  • [11]

    A.E. Al-Snafi , Int. J. Pharm. Res. Scholars 3 (2014) 663670.

  • [12]

    Y.S. Chan , L.N. Cheng, J.H. Wu, E. Chan, Y.W. Kwan, S.M.Y. Lee, G.P.H. Leung, P.H.F. Yu, S.W. Chan, Inflammopharmacology 19 (2011) 245254.

  • [13]

    F.B. Machado , R.E. Yamamoto, K. Zanoli, S.R. Nocchi, C.R. Novello, I.T.A. Schuquel, C.M. Sakuragui, H. Luftmann, T. Ueda-Nakamura, C.V. Nakamura, J.C. Palazzo de Mello, Molecules 17 (2012) 18521859.

    • Search Google Scholar
    • Export Citation
  • [14]

    Á.M. Móricz , T.T. Häbe, A. Böszörményi, P.G. Ott, G.E. Morlock, J. Chromatogr. A 1422 (2015) 310317.

  • [15]

    Á.M. Móricz , D. Krüzselyi, Á. Alberti, A. Darcsi, G. Horváth, P. Csontos, S. Béni, P.G. Ott, J. Chromatogr. A 1524 (2017) 266272.

  • [16]

    S.M. Bach , M.A. Fortuna, R. Attarian, J.T. de Trimarco, C.A.N. Catalán, Y. Av-Gay, H. Bach, Nat. Prod. Commun. 6 (2011) 163166.

  • [17]

    G. Lonergan , E. Routsi, T. Georgiadis, G. Agelis, J. Hondrelis, J. Matsoukas, L.K. Larsen, F.R. Caplan, J. Nat. Prod. 55 (1992) 225228.

  • [18]

    S. Zimmermann , S. Thomi, M. Kaiser, M. Hamburger, M. Adams, Sci. Pharm. 80 (2012) 205213.

  • [19]

    Á.M. Móricz , P.G. Ott, in: E. Tyihák (ed.), Forced-Flow Layer Chromatography, Elsevier, Amsterdam, 2016.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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