JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 31: Issue 2
Reversed-phase high-performance thin-layer chromatographic method for the simultaneous determination of glycyrrhizin and glabridin biomarkers in Glycyrrhiza glabra roots, rhizomes and herbal formulations
Authors:
Prawez Alam Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

Search for other papers by Prawez Alam in
Current site
Google Scholar
PubMed Close
,
Y.T. Kamal Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

Search for other papers by Y.T. Kamal in
Current site
Google Scholar
PubMed Close
,
Mohammed H. Alqarni Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

Search for other papers by Mohammed H. Alqarni in
Current site
Google Scholar
PubMed Close
,
Hala H. Zaatout Alexandria University, Alexandria 21215, Egypt

Search for other papers by Hala H. Zaatout in
Current site
Google Scholar
PubMed Close
, and
Maged S. Abdel-Kader Alexandria University, Alexandria 21215, Egypt

Search for other papers by Maged S. Abdel-Kader in
Current site
Google Scholar
PubMed Close
Pages:
135–142
Online Publication Date:
Apr 2018
Publication Date:
01 Apr 2018
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2018.31.2.7
Keywords:
Glycyrrhiza glabra ; Glycyrrhizin; Glabridin; High-performance thin-layer chromatography–densitometry; International Conference on Harmonization guidelines; Quantitative
Restricted access

A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 F254S HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng band−1, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug.

  • [1]

    C. Kowalchik , W.H. Hylton (eds.), Rodale’s Illustrated Encyclopedia of Herbs. Rodale Press, Emmaus, PA, 1987.

  • [2]

    I.M. Francischetti , R.Q. Monterio, J.A. Guimaraes, Biochem. Biophys. Res. Commun. 235 (1997) 259–263.

  • [3]

    E. Hasanzadeh , F. Shamsa, K. Ohtsuki, S. Rezazadeh, J. Med. Plants 9 (2010) 1–28.

  • [4]

    T. Saitoh , T. Kinoshita, S. Shibata, Chem. Pharm. Bull. 24 (1976) 752–755.

  • [5]

    L.A. Mitscher , Y.H. Park, S. Omoto, G.W. Clark, D. Clark, Heterocycles 9 (1978) 1533–1538.

  • [6]

    T. Yokota , H. Nishio, Y. Kubota, M. Mizoguchi, Pigment Cell Res. 11 (1998) 355–361.

  • [7]

    S. Tamir , M. Eizenberg, D. Somjen, S. Izrael, J. Vaya, J. Steroid Biochem. Mol. Biol. 78 (2001) 291–298.

  • [8]

    I. Binic , V. Lazarevic, M. Ljubenovic, J. Mojsa, D. Sokolovic, Evid. Based Complement. Alternat. Med. 2013 (2013) 827248; doi: 10.1155/2013/827248.

    • Search Google Scholar
    • Export Citation
  • [9]

    M. Damle , Int. J. Herb. Med. 2 (2014) 132–136

  • [10]

    O. Nerya , J. Vaya, R. Musa, S. Izrael, R. Ben-Arie, S. Tamir, J. Agric. Food Chem. 51 (2003) 1201–1207.

  • [11]

    H. Cronin , Z.D. Draelos, J. Cosmet. Dermatol. 9 (2010) 218–225.

  • [12]

    A.M. Khalil , M. Mahmoud, Handbook of the Egyptian Drugs, SpectraFlare Unlimited Technology, Cairo, 2015.

  • [13]

    S.I. Alqasoumi , P. Alam, A.J. Al-Rehaily, F. Shakeel, M.S. Abdel- Kader, J. Planar Chromatogr. 24 (2011) 48–52.

  • [14]

    P. Alam , S.I. Alqasoumi, M.S. Abdel-Kader, J. Chromatogr. Sci. 54 (2016) 58–63

  • [15]

    P. Alam , S.I. Alqasoumi, F. Shakeel, M.S. Abdel-Kader, Nat. Prod. Res. 25 (2011) 1671–1675.

  • [16]

    H.J. Yang , J.Y. Ma, J.B. Weon, C.J. Ma, Arch. Pharm. Res. 34 (2011) 1503–1508.

  • [17]

    H.J. Yang , J.Y. Ma, J.B. Weon, B. Lee, C.J. Ma, Arch. Pharm. Res. 35 (2012) 1785–1791.

  • [18]

    S. Gupta , R. Sharma, P. Pandotra, S. Jaglan, A.P. Gupta, Nat. Prod. Commun. 7 (2012) 991–994.

  • [19]

    C.S. Seo , J.A. Lee, D. Jung, H.Y. Lee, J.K. Lee, H. Ha, Arch. Pharm. Res. 34 (2011) 203–210.

  • [20]

    C.S. Seo , M.Y. Lee, H.S. Lim, S.J. Kim, H. Ha, J.A. Lee, Arch. Pharm. Res. 35 (2012) 101–108.

  • [21]

    A. Gantait , S. Pandit, Neelesh, K. Nema, P.K. Mukherjee, J. AOAC Int. 93 (2010) 492–495,

  • [22]

    P. Alam , M.F. Alajmi, N.A. Siddiqui, A.J. Al-Rehaily, O.A. Basudan, J. Coast Life Med. 2 (2014) 882–887.

  • [23]

    S.K. Chauhan , B.P. Singh, G.P. Kimothi, S. Agarwal, Indian J. Pharm Sci. 60 (1998) 251–257.

  • [24]

    B. Singh , P. Mungara, M. Nivsarkar, S. Anandjiwala, Chromatographia 70 (2009) 1665.

  • [25]

    M.J. Varsha , S.K. Uttam, S.B. Gholve, J.K. Vilasrao, Int. J. Chem- Tech. Res. 1 (2009) 826–831.

  • [26]

    International Conference on Harmonization (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use, Harmonised Triplicate Guideline on Validation of Analytical Procedures: Methodology, Recommended for Adoption at Step 4 of the ICH process on November 1996 by the ICH Steering Committee, IFPMA, Geneva.

  • Collapse
  • Expand
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2024 8 0 0
Jul 2024 32 0 0
Aug 2024 30 0 0
Sep 2024 26 0 0
Oct 2024 63 0 0
Nov 2024 73 0 0
Dec 2024 39 0 0