JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 31: Issue 2
Chromatographic determination of β-sitosterol, lupeol, and oleanolic acid in Leptadenia pyrotechnica (Forsk.) decne. – A botanical source of the ayurvedic drug Jivanti
Authors:
Raman Preet Punjabi University Patiala, 147002 Punjab, India

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Raghbir Chand Gupta Punjabi University Patiala, 147002 Punjab, India

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Saroj Kumar Pradhan Punjabi University Patiala, 147002 Punjab, India

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Pages:
150–154
Online Publication Date:
Apr 2018
Publication Date:
01 Apr 2018
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2018.31.2.9
Keywords:
High-performance thin-layer chromatography; Leptadenia pyrotechnica (Forsk.) Decne.; β-Sitosterol; Lupeol; Oleanolic acid; Rajasthan
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Among the complex mixture of biologically active compounds in Leptadenia pyrotechnica, three compounds have been used as analytical markers. A sensitive high-performance thin-layer chromatographic (HPTLC) method has been developed for the estimation. Methanolic extracts of whole plants from three populations were used on aluminum pre-coated silica gel 60 F254 plates with different mobile phases to determine the amount of β-sitosterol, lupeol, and oleanolic acid with RF value of 0.64, 0.84, and 0.47, respectively. The calibration curve was linear in the range of 2–10 μg. The method is reliable for the quantification, separation, and good resolution of these compounds from other constituents of L. pyrotechnica. To ascertain the purity of the peak from the test sample, its in-situ reflectance spectrum was compared with that from standards; the clear superimposability indicated the purity of the peaks.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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