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  • 1 Ravenshaw University, Cuttack 753 003, Odisha, India
  • | 2 Orissa University of Agriculture and Technology, Bhubaneswar 751003, Odisha
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A simple, sensitive, and precised high-performance thin-layer chromatography (HPTLC) method has been developed for the analysis of lawsone in natural vis-à-vis micropropagated plant parts of Lawsonia inermis L. Separation of the components was perfectly achieved on high-performance thin-layer chromatography (TLC) plates using optimized tertiary mobile phase of benzene‒ethyl acetate‒ acetic acid (7.5:2.5:0.1, v/v). Densitometric scanning was performed before derivatization of the plate in absorption/reflection mode, and lawsone was quantified at its maximum absorbance of wavelength of 275 nm. Linearity of the method was obtained in the concentration range of 50 to 350 ng spot−1 with a correlation coefficient (r2) of 0.9999, indicating good relationship between concentrations in opposition to the peak area. The limit of detection and limit of quantification were found to be 16 and 50 ng spot−1, respectively. The obtained recovery ranges from 95.09% to 96.90% with an average value of 96.02% proved the excellent accuracy of the method. The developed method was found to be highly sensitive, and the mobile phase enables outstanding separation of lawsone from other components present in the mixture. The International Conference on Harmonization (ICH) guidelines were followed for validation of the HPTLC method in terms of precision, repeatability, and accuracy. The maximum content of lawsone was reported in the leaves of the micropropagated plant.

  • [1]

    Anonymous, A Dictionary of Indian Raw Materials and Industrial Products, Vol. 6, CSIR, New Delhi, 1962, pp. 4750.

  • [2]

    R.B. Semwal , D.K. Semwal, S. Combrinck, C. Cartwright-Jones, A. Viljoen, J. Ethnopharmacol. 155 (2014) 80103.

  • [3]

    R. Hanna , J.N. Maciej, L. Lapinskey, L. Adamowicz, Spec. Act. 54 (1998) 10911103.

  • [4]

    C.P. Khare , Indian Medicinal Plants: An Illustrated Dictionary, Springer, New York, NY, 2007, p. 366.

  • [5]

    V.M. Gogte , Ayurvedic Pharmacology and Therapeutic Uses of Medicinal Plants Dravyagunavignyan, Chaukambha Publications, New Delhi, 2000, p. 686687.

  • [6]

    M.A. Abdulmoneim , Res. J. Biol. Sci. 2 (2007) 419423.

  • [7]

    N.X. Cuong , P.T. Binh, M.T.T. Thuy, N.H. Nami, H.L.T. Anh, N.T. Dati, N.P. Thao, P.V. Kiem, L.M. Huong, J. Chem. 47 (2009) 228232.

  • [8]

    K. Bhuvaneshwari , S.G. Poongothai, A. Kuruvilla, B. Appalaraju, Indian J. Pharmacol. 34 (2002) 260263.

  • [9]

    I.K. Makhija , D.R. Dhananjaya, S. Vijay Kumar, R. Devkar, D. Khamar, N. Manglani, S. Chandrakar, African J. Pharm. Sci. Pharm. 2 (2011) 145165.

    • Search Google Scholar
    • Export Citation
  • [10]

    P.K. Warrier , Indian Medicinal Plants a Compendium of 500 Species, Vol. 3, Orient Longman Private Limited, Chennai, 2004, pp. 303304.

  • [11]

    S. Ahmed , A. Rahman, A. Alam, M. Saleem, M. Athar, S. Sultana, J. Ethnopharmacol. 69 (2000) 157164.

  • [12]

    D.H. Bich , D.Q. Chung, B.X. Chuong, N.T. Dong, D.T. Dam, P.V. Hien, V.N. Lo, P.D. Mai, P.K. Man, D.T. Nhu, N. Tap, T. Toan, The Medicinal Plants and Animals in Vietnam, Vol. 2, Science and Technology Publishing House, Hanoi, 2004.

    • Search Google Scholar
    • Export Citation
  • [13]

    K.R. Reddy , Int. J. Crude Drug Res. 26 (1988) 137140.

  • [14]

    I. Syamsudin , H. Winarno, Res. J. Pharmacol. 2 (2008) 2023.

  • [15]

    G. Chaudhary , S. Goyal, P. Poonia, Int. J. Pharm. Sci. Drug Res. 2 (2010) 9198.

  • [16]

    G.B. Bairagi , A.O. Kabra, R.J. Mandade, Int. J. Res. Pharm. Biomed. Sci. 2 (2011) 237240.

  • [17]

    B.R. Mikhaeil , F.A. Badria, G.T. Maatooq, M.M. Amer, Z. Naturforschung. C 59 (2004) 468476.

  • [18]

    G.D. Chaudary , P. Poonia, P. Kamboj, A.N. Kalia, Int. J. Phytopharmacol. 3 (2012) 6673.

  • [19]

    S. Singh , N.M. Shrivastava, N.T. Modi, A.Q. Saifi, Curr. Sci. 51 (1982) 470471.

  • [20]

    V.K. Sharma , Tubercle 71 (1990) 293295.

  • [21]

    M. Kamal , T. Jawaid, Int. J. Biomed. Res. 2 (2010) 6268.

  • [22]

    L. Aicha , D. Rachid, A. Abed, J. Appl. Environ. Biol. Sci. 7 (2017) 92104.

  • [23]

    M. Kumar , P. Kaur, M. Chandel, A. P. Singh, A. Jain, S. Kaur, BMC Complement. Altern. Med. 17 (2017) 111.

  • [24]

    R.B. Semwal , D.K. Semwal, S. Combrinck, C. Cartwright-Jones, A. Viljoen, J. Ethnopharmacol. 155 (2014) 80103.

  • [25]

    M.M.A.A. Khan , N. Singh, K.N. Dhawan, Natl. Acad. Sci. Lett. (India) 19 (1996) 145148.

  • [26]

    B.S. Siddiqui , N. Uddin, S. Begum, Nat. Prod. Res. 23 (2009) 17401745.

  • [27]

    S. Salunke-Gawali , L. Kathawate, Y. Shinde, V.G. Puranik, T. Weyhermüller, J. Mol. Struct. 1010 (2012) 3845.

  • [28]

    E.C. Jeyaseelan , S. Jenothiny, M.K. Pathmanathan, J.P. Jeyadevan, Asian Pac. J. Trop. Biomed. 2 (2012) 798802.

  • [29]

    I. Abulyazid , E.M.E. Mahdy, R.M. Ahmed, Arabian J. Chem. 6 (2013) 265273.

  • [30]

    A.S. Oliveira , I M.C. Brighente, R.G. Lund, L.C. Llanes, R.J. Nunes, L.C. Bretanha, R. A. Yunes, P.H.A. Carvalho, J.S. Ribeiro, J. Biosci. Med. 5 (2017) 3948.

    • Search Google Scholar
    • Export Citation
  • [31]

    J.M. Sánchez-Calvo , G.R. Barbero, G. Guerrero-Vásquez, A.G. Durán, M. Macías, M.A. Rodríguez-Iglesias, J.M.G. Molinillo, F.A. Macías, Med. Chem. Res. 25 (2016) 12741285.

    • Search Google Scholar
    • Export Citation
  • [32]

    C.A. Camara , T.M.S. Silva, T.G. Da-Silva, R.M. Martins, T.P. Barbosa, A.C. Pinto, M.D. Vargas, An. Acad. Bras. Cienc. 80 (2008) 329334.

  • [33]

    L.I. López-López , S.D. Nery-Flores, S.Y. Silva-Belmares, A. Sáenz Galindo, Vitae 21 (2014) 248258.

  • [34]

    A.F.L. Vilela , B.M. Frugeri, A.L.F. Sarria, R.O.S. Kitamura, J.B. Fernandes, M.F.G.F. Silva, Q.B. Cass, C.L. Cardoso, J. Braz. Chem. Soc. 27 (2016) 534545.

    • Search Google Scholar
    • Export Citation
  • [35]

    M.M. Hasan , K.A. Nayem, A.Y.M.A. Azim, N.C. Ghosh, J. Textiles 2015 (2015) article ID: 932627.

  • [36]

    A. Ostovari , S.M. Hoseinieh, M. Peikari, S.R. Shadizadeh, S.J. Hashemi, Corrosion Sci. 51 (2009) 19351949.

  • [37]

    B.A. Mir , S.A. Mir, S. Koul, Physiol. Mol. Biol. Plants 20 (2014) 357364.

  • [38]

    K. Gupta , S. Garg, J. Singh, M. Kumar, 3 Biotech 4 (2014) 263273.

  • [39]

    A. Coste , L. Vlase, A. Halmagyi, C. Deliu, G. Coldea, Plant Cell Tiss. Organ Cult. 106 (2011) 279288.

  • [40]

    K.K. Rout , R.K. Singh, S.K. Mishra, J. Planar Chromatogr. 24 (2011) 376380.

  • [41]

    K.K. Rout , B. Das, R.K. Singh, J. Planar Chromatogr. 25 (2012) 320325.

  • [42]

    K.K. Rout , S.K. Mishra, J. Chromatogr. Sci. 52 (2014) 10891094.

  • [43]

    A. Moharana , A. Das, E. Subudhi, S.K. Naik, D.P. Barik, Proc. Natl. Acad. Sci. India (2016); doi: 10.1007/s40011-016-0740-0.

  • [44]

    N.S. El-Shaer , J.M. Badr, M.A. Aboul-Ela, Y.M. Gohar, J. Sep. Sci. 30 (2007) 33113315.

  • [45]

    A. Dhiman , K. Sharma, J. Goyal, M. Garg, A. Sharma, J. Pharm. Sci. Innov. 1 (2012) 1720.

  • [46]

    F.R. Gallo , G. Multari, G. Palazzino, G. Pagliuca, S.M.M. Zadeh, P.C.N. Biapa, M. Nicoletti, Rev. Bras. Farmacogn. 24 (2014) 133140.

  • [47]

    M.M. Patel , B.R. Solanki, N.C. Gurav, P.H. Patel, S.S. Verma, J. Adv. Pharm. Technol. Res. 4 (2013) 160165.

  • [48]

    S.S. Phirke , M. Saha, N. Chandra, Asian J. Exp. Biol. Sci. Special Issue (2010) 118120.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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