JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 31: Issue 3
Determining the contents of rupestonic acid, vitexicarpin, apigenin, and luteolin in Artemisia rupestris L. in different growth stages by thin-layer chromatographic scanning
Authors:
Dongdong Lan Xinjiang Medical University, Urumqi 830011, Xinjiang, China

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Jiajia Liu Xinjiang Medical University, Urumqi 830011, Xinjiang, China

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Xiongfei Guo Xinjiang Chinese and Minority Nationality Medicine Research Institute, Urumqi 830002, Xinjiang, China

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Guoqiang Zhu Xinjiang Chinese and Minority Nationality Medicine Research Institute, Urumqi 830002, Xinjiang, China

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, and
Shuge Tian Central Laboratory of Xinjiang Medical University, Urumqi 830011, Xinjiang, China

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Pages:
190–196
Online Publication Date:
Jun 2018
Publication Date:
01 Jun 2018
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2018.31.3.2
Keywords:
Artemisia rupestris L.; Rupestonic acid; Vitexicarpin; Apigenin; Luteolin; Thin-layer chromatographic scanning
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The contents of the four components in different growth stages of Artemisia rupestris L. was determined by thin-layer chromatographic scanning. Chloroform, methanol, formic acid, and water with the proportion of 6.35:0.63:0.17:0.07 were used as the developing solvent. The temperature was 28–32°C. The humidity was less than 30%. The scanning wavelengths were 250 nm and 352 nm. The linearity ranges of rupestonic acid, vitexicarpin, apigenin, and luteolin were 0.128–0.725, 0.0706–0.400, 0.0255–0.145, and 0.0172–0.0976 μg, respectively. The components had the corresponding correlation coefficients of 0.9909, 0.9908, 0.9971, and 0.9934, which showed a good linear relationship. Precision analyses showed a relative standard deviation of <5.0%. Stability studies showed that rupestonic acid, vitexicarpin, apigenin, and luteolin can be stable for at least 30 min at room temperature. Their average recovery percentages were 99.9%, 103.6%, 98.07%, and 99.9%, respectively. The contents of the four components in the different growth stages of A. rupestris L. were studied. The obvious changes of the four components could be used as the control for the quality of A. rupestris L. and the determination of the time of harvest.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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