JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 31: Issue 3
Interspecies estimation of β-sitosterol by a validated high-performance thin-layer chromatography method in genus Ficus and cytotoxic activity against HepG2, HEK-293, MCF-7, and MDA-MB-231 cell lines
Authors:
Perwez Alam King Saud University, Riyadh, Saudi Arabia

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Tawfeq A. Alhowiriny King Saud University, Riyadh, Saudi Arabia

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Nasir A. Siddiqui King Saud University, Riyadh, Saudi Arabia

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Saleh I. Alqasoumi King Saud University, Riyadh, Saudi Arabia

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Omer A. Basudan King Saud University, Riyadh, Saudi Arabia

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Azmat Ali Khan King Saud University, P.O. Box 2457, Riyadh-11451, Saudi Arabia

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Abdullah T. Alhowiriny King Saud University, Riyadh, Saudi Arabia

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Nawazish Alam Jazan University, Jazan, Saudi Arabia

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Pages:
213–219
Online Publication Date:
Jun 2018
Publication Date:
01 Jun 2018
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2018.31.3.6
Keywords:
Ficus species; β-Sitosterol; High-performance thin-layer chromatography; Validation; Methylthiazol tetrazolium assay
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Extensive research on Ficus species has shown their excellent cytotoxic potential which motivated the authors for further evaluation of its other species. In this article, the β-sitosterol content in the chloroform extract of the leaves of five Ficus species (Ficus carica [FCCE], Ficus nitida [FNCE], Ficus ingens [FICE], Ficus palmata [FPCE], and Ficus vasta [FVCE]) was estimated by a validated high-performance thin-layer chromatography (HPTLC) method along with cytotoxic activity. The chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with hexane and ethyl acetate (8:2, v/v) as the mobile phase. The developed plate was derivatized with p-anisaldehyde reagent, scanned, and quantified at λ = 550 nm. It furnished a compact and intense peak of β-sitosterol at RF = 0.17 ± 0.001. The contents of β-sitosterol (μg mg−1 of the dried weight of the extract) in the selected Ficus species were found as: FCCE (1.047 μg mg−1) > FVCE (0.771 μg mg−1) > FNCE (0.372 μg mg−1) > FPCE (0.309 μg mg−1), while it was absent in F. ingens. Methylthiazol tetrazolium (MTT) assay was used to compare the cytotoxic potential of all Ficus species against HepG2 (liver), HEK-293 (kidney), MCF-7 (breast), and MDA-MB 231 (breast) cell lines. The FCCE exhibited good cytotoxic property against HepG2, HEK-293, and MDA-MB-231 cells (IC50: 32.5, 41.4, and 47.3 μg mL−1, respectively), while FICE showed against HepG2 and MDA-MB-231 cells (IC50: 31.4 and 41.2 μg mL−1, respectively). The remaining Ficus extracts were found to be very less effective or insignificant. The cytotoxic property of FCCE is also supported by the HPTLC estimation of β-sitosterol which is reported to exhibit anticancer properties by interfering with multiple cell signaling pathways, including cell cycle, apoptosis, and proliferation. Our data suggest that the developed HPTLC method can be further employed in the analysis of marketed herbal formulations, and the active Ficus species can be further subjected to isolation of cytotoxic phytoconstituents.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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