View More View Less
  • 1 Saurashtra University, Rajkot, Gujarat, India
Restricted access

Salvadora persica plant having a number of antimicrobial substances and the roots of the S. persica shrub have been demonstrated to possess antimicrobial activity. Sticks from the roots of S. persica have been used for centuries as a traditional method of cleaning teeth. Benzyl isothiocyanate is the main antimicrobial agent found in the root of the plant. Here, we developed high-performance thin-layer chromatography (HPTLC) and reversed-phase high-performance liquid chromatography (RP-HPLC) methods for the qualitative analysis of benzyl isothiocyanate in the S. persica root powder and the dental care herbal formulation labeled to contain Miswak extract. A HPTLC method was carried out using pre-coated silica gel aluminum TLC plates 60 F254, and the mobile phase consisting of n-hexane (10 mL) furnished compact spots at RF 0.39 ± 0.01. A HPLC method was carried out using a column Phenomenex C18(150 mm × 4.6 mm, 5 μm) and methanol‒10 mm ammonium acetate (70:30, v/v) as the mobile phase with a good retention time, 8.179 ± 0.05. The developed method was validated by HPTLC in accordance with the International Council of Harmonization (ICH) Q2 (R1) guidelines for precision, accuracy, and robustness. The developed plate was scanned and quantified densitometrically at 250 nm. Linear regression analysis revealed a good linear relationship between the peak area and the amount of benzyl isothiocyanate in the range of 3–15 μg per band (r2 = 0.997). Fingerprinting was done by HPTLC and HPLC method by using a dental care herbal formulation labeled to contain Miswak extract. The proposed method will be useful to evaluate the therapeutic efficacy of Miswak extract and dental care herbal formulations containing Miswak extract.

  • [1]

    M. Khatak , S. Khatak, A.A. Siddqui, N. Vasudeva, A. Aggarwal, P. Aggarwal, Pharmacogn. Rev. 4 (2010) 209214.

  • [2]

    S.K. Hordofa , The use of chewing sticks and oral health, University of Oslo, 2016;

    • Search Google Scholar
    • Export Citation
  • [3]

    B. H. Aboul-Enein , Saudi J. Dent. Res. 5 (2014) 913.

  • [4]

    G. Brüsewitz , L.F. Chasseaud, B.D. Cameron, W.H. Mennicke, Biochem. J. 162 (1976) 99107.

  • [5]

    M.M. Haque , S.A. Alsareii, Saudi Med. J. 36 (2015) 530543.

  • [6]

    H.R. Poureslami , A. Makarem, F. Mojab, Dent. Res. J. 4 (2007) 106110.

  • [7]

    H. Ahmad , K. Rajagopal, Med. Aromat. Plants 2 (2013) 15.

  • [8]

    V. Agrawal , S. Kapoor, I. Agrawal, J. Diet. Suppl. 14 (2017) 229240.

For Submission Guideline click HERE

Manuscript Submission: JPC Manuscript Submission

Senior editors

Editor(s)-in-Chief: B. Spangenberg

Managing Editor: Kakuk Ágnes

Editorial Board

  • T.H. Dzido (Lublin, Poland)
  • Á. M. Móricz (Budapest, Hungary)
  • C.F. Poole (Detroit, MI, USA)
  • E. Reich (Muttenz, Switzerland)
  • B. Renger (Radolfzell, Germany)
  • I. Vovk (Ljubljana, Slovenia)


Advisory Board

  • E. BODOKI (Cluj-Napoca, Romania)
  • U. A. TH. BRINKMAN (Amsterdam, The Netherlands)
  • I. CHOMA (Lublin, Poland)
  • V. COMAN (Cluj-Napoca, Romania)
  • W. DAMMERTZ (Kressbronn, Germany)
  • K. FERENCZI-FODOR (Budapest, Hungary)
  • G. INDRAYANTO (Surabaya, Indonesia)
  • R. E. KAISER (Bad Dürkheim, Germany)
  • I. KLEBOVICH (Budapest, Hungary)
  • T. KOWALSKA (Katowice, Poland)
  • L. LEPRI (Florence, Italy)
  • E. MINCSOVICS (Budapest, Hungary)
  • D. NUROK (Indianapolis, IN, USA)
  • B. RENGER (Radolfzell, Germany)
  • J. K. RÓŻYŁO (Lublin, Poland)
  • J. SHERMA (Easton, PA, USA)
  • M. WAKSMUNDZKA-HAJNOS (Lublin, Poland)
  • D. WEDGE (Mississippi, USA)

A. Kakuk - Managing Editor, Akadémiai Kiadó
Address: P.O. Box 245. H-1519 Budapest, Hungary
Fax: +36 1 464 82 95

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences


Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
per Year
per Year
Founder Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Feb 2021 0 0 0
Mar 2021 13 0 0
Apr 2021 8 0 0
May 2021 11 0 0
Jun 2021 4 0 0
Jul 2021 15 0 0
Aug 2021 0 0 0