JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 31: Issue 6
Design-of-experiment approach for the development and validation of a high-performance thin-layer chromatography method for the simultaneous estimation of berberine chloride and galangin in Tinospora cordifolia M. and Alpinia galanga L. and their herbal formulations
Authors:
Rajendra K. Patel K.B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat 382023, India

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and
Priti D. Trivedi K.B. Institute of Pharmaceutical Education and Research, Gandhinagar, Gujarat 382023, India

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Pages:
451–459
Online Publication Date:
Dec 2018
Publication Date:
01 Dec 2018
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2018.31.6.5
Keywords:
Design-of-experiment; Box–Behnken design; Berberine chloride; Galangin
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In the present study, a design-of-experiment (DoE) approach Was used to determine optimized mobile-phase compositions for the development of a high-performance thin-layer chromatography (HPTLC) method for the simultaneous estimation of berberine chloride (BER-H) and galangin (GAL) in Tinospora cordifolia M. and Alpinia galanga L., respectively, and their formulations. A Box‒Behnken design (BBD) was used to optimize the compositional parameters and evaluate the main effect, interaction effects and quadratic effects of the mobile-phase compositions on the retardation factor (RF) of both drugs. HPTLC separation was performed on aluminum plates pre-coated with silica gel 60 F254 as the stationary phase, using toluene‒ethyl acetate‒formic acid (3:6:1, v/v) as the mobile phase at a wavelength of 267 nm. A sharp and well-resolved peak was obtained for BER-H and GAL at RF values of 0.17 ± 0.01 and 0.82 ± 0.01, respectively. The calibration curve was in the range of 200–1200 ng per band for both BER-H and GAL, with r2 = 0.984 and r2 = 0.980, respectively. Statistical insight was achieved with analysis of variance (ANOVA). The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification, robustness, and specificity. To provide a better visualization of the statistically significant factors derived from the statistical analysis, the perturbation plot and response surface plot for the effect of independent variables on the RF of BER-H and GAL were evaluated. The developed HPTLC method was found to be simple, accurate, precise, sensitive, and specific for the simultaneous quantification of berberine chloride and galangin in Tinospora cordifolia M. and Alpinia galanga L., respectively, and their formulations.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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