Authors:
Jyotsana Dwivedi CSIR - National Botanical Research Institute, Lucknow, India
Banasthali Vidyapeeth, Banasthali, India

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Abhishek Gupta Pranveer Singh Institute of Technology, Kanpur, India

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Shikhar Verma Amity University, Lucknow, India

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Sarvesh Paliwal Banasthali Vidyapeeth, Banasthali, India

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Ajay Kumar Singh Rawat CSIR - National Botanical Research Institute, Lucknow, India

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A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination and validation of ursolic acid, β-sitosterol, lupeol, and quercetin in the methanolic fraction of Ichnocarpus frutescens L. was developed for the first time. For achieving good separation, a mobile phase of toluene‒ethyl acetate‒formic acid (8:2:0.1, v/v) was used. Densitometric determination was carried out at 500 nm for ursolic acid, 550 nm for β-sitosterol, 650 nm for lupeol, and 310 nm for quercetin in reflection–absorption mode, and the calibration curves were linear in the range of 100–600 ng per spot. During the analysis, the methanolic fraction of I. frutescens L. showed the presence of ursolic acid (0.34%), β-sitosterol (0.27%), lupeol (0.27%), and quercetin (0.26%). The proposed method is simple, precise, specific, and accurate. The obtained data can be used for routine analysis of reported biomarkers in crude drug and extracts. The simultaneous quantification and method validation of these biomarkers have not yet been reported in I. frutescens L., which may be utilized for the proper standardization of the plant.

  • [1]

    Anonymous, Wealth of India, Raw Materials, Council of Scientific and Industrial Research (CSIR), New Delhi, 1976, p. 16263.

  • [2]

    M. Parinitha , G.U. Harish, N.C. Vivek, T. Mahesh, M.B. Shivanna, Indian J. Tradit. Knowl. 31 (2004) 3750.

  • [3]

    G. Du Thie , A. Crozier, Curr. Opin. Nutr. Metab. 3 (2000) 447451.

  • [4]

    C.T. Kumarappan , S.C. Mandal, Exp. Oncol. 29 (2007) 94101.

  • [5]

    C.T. Kumarappan , E. Thilagam, C. Subhash Mandal, Saudi J. Biol. Sci. 19 (2012) 349355.

  • [6]

    P.J. Ferguson , E. Kurowska, D.J. Freeman, A.F. Chambers, D.J. Koropatnick, J Nutr 134 (2004) 152935.

  • [7]

    E.A. Hudson , P.A. Dinh, T. Kokubun, M.S.J. Simmonds, A. Gescher, Cancer Epidemiol. Biomarkers Prev. 9 (2000) 116370.

  • [8]

    A. Chatterjee , S.C. Pakrashi, The Treatise on Indian Medicinal Plants, Publications and Information Directorate, New Delhi, 1995, p. 110112.

    • Search Google Scholar
    • Export Citation
  • [9]

    K.M. Nadkarni , The Indian Materia Medica, Bombay Popular Prakashan, Bombay, 1982, p. 674.

  • [10]

    J. Anbu , S. Suman, K.S.L.V.V.S.N. Swaroop Kumar, R. Satheeshkumar, S. Nithya, R. Kannadhasan, J. Pharm. Sci. Res. 3 (2011) 11821189.

  • [11]

    C. Kumarappan , S.C. Mandal, Indian J. Physiol. Pharmacol. 58 (2014) 441445.

  • [12]

    C.T. Kumarappan , R. Chandra, S.C. Mandal, Pharmacol. Online 3 (2006) 20116.

  • [13]

    A. Pandurangan , R. Khosa, S. Hemalatha, DARU J. Pharm. Sci. 17 (2009) 15.

  • [14]

    T. Chidambaram , T. Kumarappan, N. Rao, S.C. Mandal, J. Cell Mol. Biol. 6 (2007) 175187.

  • [15]

    C. Kumarappan , S.C. Mandal, Med. Chem. Res. 17 (2008) 219233.

  • [16]

    R. Barik , S. Jain, D. Qwatra, A. Joshi, G.S. Tripathi, R. Goyal, Indian J. Pharmacol. 40 (2008) 1922.

  • [17]

    S. Neha , T. Mani, T. Prakash, D. Prashant, Indian J. Nov. Drug Deliv. 4 (2012) 2427.

  • [18]

    Anonymous, A dictionary of Indian raw materials and industrial products, Vol. 3, NISCOM, New Delhi, 2002, p. 330.

  • [19]

    S.P. Ambasta , Useful plants of India, NISCOM, New Delhi, 1999, p. 283.

  • [20]

    R.P. Singh , R.P. Singh, J. Indian Chem. Soc. 14 (1987) 715756.

  • [21]

    D.K.M. Lakshmi , E. Venkata Rao, D. Venkata Rao, Indian Drugs 22 (1985) 552553.

  • [22]

    N.K. Singh , V. Singh, Chin. J. Nat. Med. 10 (2012) 241246.

  • [23]

    P.K. Minchona , R.N. Tandon, Phytochemistry 19 (1980) 20532055.

  • [24]

    K. Chaudhary , B. Aggarwal, R.K. Singla, Indo Global J. Pharm. Sci. 2 (2012) 6369.

  • [25]

    M. Daniel , S.D. Sabnis, Indian J. Exp. Biol. 16 (1978) 512513.

  • [26]

    M.S.Y. Khan , K. Javed, M.H. Khan, Br. J. Chem. Soc. 72 (1995) 6566.

  • [27]

    R.P. Singh , J, Indian Chem. Soc. 64 (1987) 715716.

  • [28]

    B. Aggarwal , Md. Ali, V. Singh, R.K. Singla, Chin. J. Nat. Med. 8 (2010) 401404.

  • [29]

    D. Mammen , M. Daniel, R.T. Sane, Pharmacogn. J. 2 (2011) 712.

  • [30]

    M.P. Jeewandara , P.L. Hettiarachchi, A.M. Abeysekera, J. Med. Plants Stud. 5 (2017) 249254.

  • [31]

    V.S. Joshi , V.R. Patil, A.N. Avalaskar, RJPBCS 2 (2011) 558563.

  • [32]

    ICH-Q2A, Text on Validation of Analytical Procedures, Harmonized Tripartite Guideline prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Geneva, 1994.

  • [33]

    ICH-Q2B, Validation of Analytical Procedures: Methodology, Harmonized Tripartite Guideline prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Geneva, 1996.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
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Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
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H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
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Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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