JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 32: Issue 2
Validated simultaneous high-performance thin-layer chromatographic analysis of ursolic acid, β-sitosterol, lupeol and quercetin in the methanolic fraction of ichnocarpus frutescens
Authors:
Jyotsana Dwivedi CSIR - National Botanical Research Institute, Lucknow, India
Banasthali Vidyapeeth, Banasthali, India

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,
Abhishek Gupta Pranveer Singh Institute of Technology, Kanpur, India

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,
Shikhar Verma Amity University, Lucknow, India

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Sarvesh Paliwal Banasthali Vidyapeeth, Banasthali, India

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, and
Ajay Kumar Singh Rawat CSIR - National Botanical Research Institute, Lucknow, India

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Pages:
103–108
Online Publication Date:
Apr 2019
Publication Date:
01 Apr 2019
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2019.32.2.4
Keywords:
β-Sitosterol; High-performance thin-layer chromatography; Ichnocarpus frutescens ; Lupeol; Quercetin; Ursolic acid
Restricted access

A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination and validation of ursolic acid, β-sitosterol, lupeol, and quercetin in the methanolic fraction of Ichnocarpus frutescens L. was developed for the first time. For achieving good separation, a mobile phase of toluene‒ethyl acetate‒formic acid (8:2:0.1, v/v) was used. Densitometric determination was carried out at 500 nm for ursolic acid, 550 nm for β-sitosterol, 650 nm for lupeol, and 310 nm for quercetin in reflection–absorption mode, and the calibration curves were linear in the range of 100–600 ng per spot. During the analysis, the methanolic fraction of I. frutescens L. showed the presence of ursolic acid (0.34%), β-sitosterol (0.27%), lupeol (0.27%), and quercetin (0.26%). The proposed method is simple, precise, specific, and accurate. The obtained data can be used for routine analysis of reported biomarkers in crude drug and extracts. The simultaneous quantification and method validation of these biomarkers have not yet been reported in I. frutescens L., which may be utilized for the proper standardization of the plant.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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