Determination of sugar biomarkers in methanol root extracts of Asparagus species by high-performance thin-layer chromatography

Manish Kapoor; Raghbir Chand Gupta; Pooja Mawal; Saroj Kumar Pradhan

doi:10.1556/1006.2019.32.2.5

JPC - Journal of Planar Chromatography - Modern TLC

Volume/Issue:: Volume 32: Issue 2

Determination of sugar biomarkers in methanol root extracts of Asparagus species by high-performance thin-layer chromatography

Authors:

Manish Kapoor

Manish Kapoor Punjabi University Patiala, Punjab 147002, India

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Raghbir Chand Gupta

Raghbir Chand Gupta Punjabi University Patiala, Punjab 147002, India

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Pooja Mawal

Pooja Mawal Punjabi University Patiala, Punjab 147002, India

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Saroj Kumar Pradhan

Saroj Kumar Pradhan Punjabi University Patiala, Punjab 147002, India

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Pages:: 109–114

Online Publication Date:: Apr 2019

Publication Date:: 01 Apr 2019

Article Category:: Research Article

DOI:: https://doi.org/10.1556/1006.2019.32.2.5

Keywords:: Asparagus ; High-performance thin-layer chromatography; Fructose; Sucrose; Glucose

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This study is a first attempt to present a report about a high-performance thin-layer chromatography (HPTLC)–densitometric method that has been developed and validated for the quantification of sugars, i.e., glucose, fructose, sucrose, maltose, and raffinose from the methanol extracts of roots of 7 Asparagus species ‒ A. adscendens, A. racemosus, A. retrofractus, A. officinalis, A. densiflorus, A. falcatus, and A. sprengeri. The separation and quantification of sugars were achieved using the mobile phase propanol‒ethyl acetate‒ water (6:3:1, v/v). Derivatization of the bands was done by using diphenylamine, aniline, and ortho-phosphoric acid reagents, and densitometric scanning was done at 600 nm in absorbance–reflectance mode. This method gave the best resolution of bands at R_F = 0.55, 0.50, 0.31, 0.11, and 0.46, corresponding to fructose, glucose, maltose, raffinose, and sucrose, respectively. The calibration curves were linear in the range of 100–500 ng per spot. The limit of detection (LOD) and the limit of quantification (LOQ) ranged between 12.77–75.48 ng and 38.69–228.73 ng, respectively. The intraand inter-day precisions were found between 0.33–1.46 in terms of relative standard deviation (RSD). The average recoveries ranged between 97.84 –99.91%. The validated method was successfully used to detect and quantify sugars in the methanol root extracts of the 7 Asparagus species.

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JPC - Journal of Planar Chromatography - Modern TLC

Print ISSN:: 0933-4173

Online ISSN:: 1789-0993

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Journal of Planar Chromatography - Modern TLC
Language	English
Size	A4
Year of Foundation	1988
Volumes per Year	1
Issues per Year	6
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0933-4173 (Print)
ISSN	1789-0993 (Online)