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Ahmed I. FoudahPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Prawez AlamPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Abdulaziz I. Al FuraihPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Mohammad Ayman A. SalkiniPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Maged S. Abdel-KaderPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia
Alexandria University, Alexandria 21215, Egypt

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Fenugreek is one of the oldest medicinal plants known by mankind. The aim of this study is to evaluate the effect of environmental conditions on the saponin contents of the plant. The amount of diosgenin in different samples of fenugreek was estimated by high-performance thin-layer chromatography (HPTLC). The developed method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. HPTLC was carried out using hexane‒acetone (8:2%, v/v) on 20 cm × 10 cm glass coated silica gel 60 F254 plates. The developed plates were scanned and quantified densitometrically at λ = 430 nm. Linear regression analysis revealed a good linear relationship between the area under the peak and the amount of diosgenin in the range of 50–400 ng per band. The amount of diosgenin which reflects the total saponin contents varied according to the country of origin. The sample obtained from Yemen showed the highest amount of diosgenin followed by the Saudi sample. Both locations represent areas with the highest altitude (Yemen) and the highest temperature (Saudi).

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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