JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 32: Issue 5
Development and Validation of a High-Performance Thin-Layer Chromatography Method for the Quantification of Rutin in the Fruit Pulp of Benincasa hispida (Thunb.) Cogniaux
Authors:
Anshul Shakya Dibrugarh University, Dibrugarh 786 004, Assam, India

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Neelutpal Gogoi Dibrugarh University, Dibrugarh 786 004, Assam, India

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Sushil Kumar Chaudhary University of the Free State, Bloemfontein 9300, South Africa

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Hans Raj Bhat Dibrugarh University, Dibrugarh 786 004, Assam, India

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Surajit Kumar Ghosh Dibrugarh University, Dibrugarh 786 004, Assam, India

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Pages:
371–377
Online Publication Date:
Oct 2019
Publication Date:
01 Oct 2019
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2019.32.5.4
Keywords:
High-performance thin-layer chromatography; Quality control; Benincasa hispida ; Rutin; Fingerprinting profile; Standardization
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Summary

Background: The fruits of Benincasa hispida (Thunb.) Cogniaux, a ‘rasayana’ in ‘Ayurveda’, are enriched with several secondary metabolites, and rutin is one of them. Fruits are used for their anabolic, brain tonic, carminative, diuretic, memory enhancer, refrigerant, and vitalizer properties. Objective: In view of the fact that herbal medicines and/or products are facing challenges towards global acceptance due to the lack of universally accepted standardization method (s), the aim of the current investigation was to develop and validate a high-performance thin-layer chromatography (HPTLC)-densitometry method for the quantification of rutin in the hydroalcoholic extracts of the fruit pulp of B. hispida (HABH). Materials and methods: The separation was achieved in a solvent system consisting of ethyl acetate-formic acid-acetic acid-water at a ratio of 7.2:0.7:0.7:1.4 by volume on a TLC aluminum plate pre-coated with silica gel 60 F254. Quantifications were performed by densitometric scanning under a deuterium lamp at a wavelength of 268 nm in the absorbance mode. The precision, accuracy, and reproducibility of the HPTLC method were validated by the International Conference on Harmonization (ICH) guidelines. Results: The mobile phase employed for HPTLC/TLC resulted in good separation for rutin (RF = 0.357). The limit of detection and limit of quantification of the analysis were found to be 0.1 and 0.3 µg per band, respectively. The rutin concentration in the HABH was found to be 178.28 ± 3.62 µg in 10 mg of the extract. Conclusion: The method developed here is simple, fast, reliable, and sensitive and can be implemented in the analysis and routine quality control of B. hispida formulations containing rutin.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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