JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 32: Issue 5
Development of a Reversed-Phase High-Performance Thin-Layer Chromatography Method for the Simultaneous Determination of Trigonelline and Diosgenin Biomarkers in Trigonella foenum-graecum L. Seeds Grown in Variable Environment
Authors:
Ahmed I. Foudah Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Prawez Alam Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Mohammed H. Alqarni Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Mohammad Ayman A. Salkini Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Maged S. Abdel-Kader Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia
Alexandria University, Alexandria 21215, Egypt

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Pages:
379–384
Online Publication Date:
Oct 2019
Publication Date:
01 Oct 2019
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2019.32.5.5
Keywords:
Trigonelline; Diosgenin; Reversed-phase high-performance thin-layer chromatography-densitometry; ICH guidelines
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Summary

We have recently reported on the effect of the environmental conditions on the quantity of diosgenin. Attempts for the simultaneous quantification of trigonelline and diosgenin using normal-phase silica gel plates were not successful. A high-performance thin-layer chromatography (HPTLC) method was developed using glass-backed plates coated with RP-18 silica gel 60 F254S and acetonitrile-water (7.5:2.5, V/V) as the mobile phase. Trigonelline and diosgenin peaks were well separated with RF values 0.29 ± 0.02 and 0.17 ± 0.01, respectively. The TLC plates were directly scanned at 267 nm for trigonelline and at 430 nm after derivatization with vanillin-sulfuric acid for diosgenin. Linear regression analysis revealed a good linear relationship between the peak area and the amounts of trigonelline and diosgenin in the range of 200–1400 and 50–300 ng per band, respectively. The method was validated in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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