Authors:
Ahmed I. FoudahPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Prawez AlamPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Mohammed H. AlqarniPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Mohammad Ayman A. SalkiniPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia

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Maged S. Abdel-KaderPrince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia
Alexandria University, Alexandria 21215, Egypt

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Summary

We have recently reported on the effect of the environmental conditions on the quantity of diosgenin. Attempts for the simultaneous quantification of trigonelline and diosgenin using normal-phase silica gel plates were not successful. A high-performance thin-layer chromatography (HPTLC) method was developed using glass-backed plates coated with RP-18 silica gel 60 F254S and acetonitrile-water (7.5:2.5, V/V) as the mobile phase. Trigonelline and diosgenin peaks were well separated with RF values 0.29 ± 0.02 and 0.17 ± 0.01, respectively. The TLC plates were directly scanned at 267 nm for trigonelline and at 430 nm after derivatization with vanillin-sulfuric acid for diosgenin. Linear regression analysis revealed a good linear relationship between the peak area and the amounts of trigonelline and diosgenin in the range of 200–1400 and 50–300 ng per band, respectively. The method was validated in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness.

  • [1]

    G.A. Petropoulos , Fenugreek, the Genus Trigonella, Medicinal and Aromatic Plants – Indutsrial Profiles, TJ International Ltd., Padstow, Cornwall, 2002.

    • Search Google Scholar
    • Export Citation
  • [2]

    M. Yoshikawa , T. Murakami, H. Komatsu, N. Murakami, J. Yamahára, H. Matsuda, Chem. Pharm. Bull. (Tokyo) 45 (1997) 8187.

  • [3]

    M. Howard , Traditional folk remedies: a comprehensive herbal, Century Hutchinson Ltd., London, 1987, pp. 134138.

  • [4]

    C. Ulbricht , E. Basch, D. Burke, L. Cheung, E. Ernst, N. Giese, I. Foppa, P. Hammerness, S. Hashmi, G. Kuo, M. Miranda, S. Mukherjee, M. Smith, D. Sollars, S. Tanguay-Colucci, N. Vijayan, W. Weissner, J. Herb. Pharmacother. 7 (2007) 143177.

    • Search Google Scholar
    • Export Citation
  • [5]

    U. Winterfeld , Y. Meyer, A. Panchaud, A. Einarson, Breastfeed Med. 7 (2012) 317318.

  • [6]

    M. Mortel , S.D. Mehta, J. Hum. Lact. 29 (2013) 154162.

  • [7]

    P. Khosla , D.D. Gupta, R.K. Nagpal, Indian J. Pharmacol. 27 (1995) 8993.

  • [8]

    R.D. Sharma , A. Sarkar, D.K. Hazra, B. Misra, J.B. Singh, B.B. Maheshwari, Phytother. Res. 10 (1996) 519520.

  • [9]

    Y. Sauvaire , G. Ribes, J.C. Baccou, M.M. Loubatieères-Mariani, Lipids 26 (1991) 191197.

  • [10]

    G.S. Sidhu , D.G. Oakenfull, Br. J. Nutr. 55 (1986) 643649.

  • [11]

    M. Al-Habori , A. Raman, M.J. Lawrence, P. Skett, Int. J. Exp. Diabetes Res. 2 (2001) 9199.

  • [12]

    J. Raju , J.M. Patlolla, M.V. Swamy, C.V. Rao, Cancer Epidemiol. Biomarkers Prev. 13 (2004) 13921398.

  • [13]

    F. Li , P.P. Fernandez, P. Rajendran, K.M. Hui, G. Sethi, Cancer Lett. 292 (2010) 197207.

  • [14]

    J.F. Feng , Y.N. Tang, H. Ji, Z.G. Xiao, L. Zhu, T. Yi, Oxid. Med. Cell Longev. 2017 (2017) 4176518, 1-9; DOI: 10.1155/2017/4176518.

  • [15]

    J. Zhou , L. Chan, S. Zhou, Curr. Med. Chem. 19 (2012) 35233531.

  • [16]

    O. Yoshinari , K. Igarashi, Curr. Med. Chem. 17 (2010) 2196202.

  • [17]

    S.N. Shah , S.L. Bodhankar, S.L. Badole, H.V. Kamble, V. Mohan, Cell Tissue Res. 6 (2006) 585590.

  • [18]

    A.I. Foudah , P. Alam, A.I. Al Furaih, M.A. Salkini, M.S. Abdel-Kader, J. Planar Chromatogr. 32 (2019) 8993.

  • [19]

    M.H. Alqarni , P. Alam, A.M. Al Kharji, M.A. Salkini, M.S. Abdel-Kader, Curr. Pharm. Anal. (article ahead of print).

  • [20]

    International Conference on Harmonization (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use, Harmonized Triplicate Guideline on Validation of Analytical Procedures: Text and Methodology Q2 (R1), Complementary Guideline on Methodology incorporated in November 2005 by the ICH Steering Committee, IFPMA, Geneva.

    • Search Google Scholar
    • Export Citation
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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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