JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 32: Issue 5
Quantification of Lupeol in Polyherbal Formulation by High-Performance Thin-Layer Chromatography Method Using Design of Experiments as a Tool to Assess Optimization of Method Development and Extraction Efficiency
Authors:
Veena Patel Anand Pharmacy College, Opp. Town Hall, Anand, Gujarat, India

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Kinjal Solanki Anand Pharmacy College, Opp. Town Hall, Anand, Gujarat, India

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Kalpana Patel Anand Pharmacy College, Opp. Town Hall, Anand, Gujarat, India

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Tejal Gandhi Anand Pharmacy College, Opp. Town Hall, Anand, Gujarat, India

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Pages:
385–392
Online Publication Date:
Oct 2019
Publication Date:
01 Oct 2019
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2019.32.5.6
Keywords:
High-performance thin-layer chromatography; Lupeol; Polyherbal formulation; Full factorial design; Box-Behnken design; Method development; Validation
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Summary

A simple, specific, and quantitative high-performance thin-layer chromatographic (HPTLC) method has been developed for the quantitative determination of lupeol in 2 marketed formulations, namely, Manasamitra vatakam and Amree plus capsule. Chromatographic development was performed by using a pre-coated silica gel 60 F254 aluminum-backed plate, and the development was carried out using toluene-ethyl acetate (9.48:0.52, V/V) as the optimized mobile phase. The developed TLC plates were derivatized by using anisaldehyde-sulfuric acid reagent. The detection of lupeol was carried out at 600 nm. Box-Behnken design was applied for optimization of the chromatographic conditions, and combinations of factors, such as mobile phase composition (volume of ethyl acetate) (A), chamber saturation time (B), and migration distance (C) likely to affect RF were identified from preliminary trials and further optimized using a response surface design. Among 3 factors, the significant factor found was the volume of ethyl acetate that resulted in higher change in the RF value and can be considered as a critical method parameter. Full factorial design was applied for optimization of extraction efficiency. The factors selected for the optimization process were volume of methanol (A) and duration of extraction (B) with percentage yield of extract as response. The linear ranges were found to be 500–3000 ng per band. The accuracy and precision measured were less than 2% relative standard deviation for lupeol. The sensitivity of the method in terms of the limit of detection (LOD) and the limit of quantification (LOQ) was measured. The proposed method was found to be accurate, precise, reproducible, robust, and specific and can be applicable for the determination of lupeol in the quality-control testing of extract and polyherbal formulations.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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