View More View Less
  • 1 Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China
  • | 2 Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai, China
Restricted access

Summary

A thin-layer chromatography (TLC)–bioautography–mass spectrometry (MS) method coupled with a liquid chromatography–MS-controlled autopurification system was developed and applied for screening and isolating natural dipeptidyl peptidase IV (DPP IV) inhibitor from plant extracts. An unknown constituent with potential DPP IV inhibitory activity from bulbs of Fritillaria cirrhosa was discovered using TLC–bioautography, followed by using a TLC interface with mass spectrometry to obtain m/z of the target compound, and the purification of the compound was directly achieved with a mass-directed autopurification system for 40 runs of injection. Finally, 2.1 mg of the compound was obtained and identified as peimisine by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. The half maximal inhibitory concentration (IC50) of DPP IV inhibitory activity was determined at 80.5 µm comparing to 58.0 µm of the standard DPP IV inhibitor diprotin A by a spectrophotometric method.

  • [1]

    F. He , Y. He, X. Zheng, R. Wang, J. Lu, Z. Dai, S. Ma, J. AOAC Int. 101 (2018) 686694.

  • [2]

    C. Oellig , T. Melde, J. Chromatogr. A 1441 (2016) 12633.

  • [3]

    L.-X. Fang , A.-Z. Xiong, X. Yang, W.-Z. Cheng, Z.-T. Wang, J. Sep. Sci. 37 (2014) 20322038.

  • [4]

    S. Dewanjee , M. Gangopadhyay, N. Bhattacharya, R. Khanra, T.K. Dua, J. Pharm. Anal. 5 (2015) 7584.

  • [5]

    S.K. El Euch , L. Cieśla, N. Bouzouita, J. AOAC Int. 97 (2014) 12911298.

  • [6]

    M.O. Hamburger , G.A. Cordell, J. Nat. Prod. 50 (1987) 1922.

  • [7]

    M. Olech , L. Komsta, R. Nowak, Ł. Cieśla, M. Waksmundzka-Hajnos, Food Chem. 132 (2012) 549553.

  • [8]

    Y.-B. Yang , L.-H. Gu, Y. Xiao, Q. Liu, H.-J. Hu, Z.-T. Wang, K.-X. Chen, PLOS ONE 10 (2015) e0116922; DOI: 10.1371/journal.pone.0116922.

  • [9]

    J. Tang , J. Zhou, Q. Tang, T. Wu, Z. Cheng, Phytochem. Anal. 27 (2016) 512.

  • [10]

    L.-H. Gu , L.-P. Liao, H.-J. Hu, S.W.A. Bligh, C.-H. Wang, G.-X. Chou, Z.-T. Wang, J. Chromatogr. A 1411 (2015) 116122.

  • [11]

    Q.-J. Zhang , Z.-F. Zhang, D.-Q. Yu, J. Asian Nat. Prod. Res. 13 (2011) 10981103.

  • [12]

    L. Zhang , J. Shi, J. Tang, Z. Cheng, X. Lu, Y. Kong, T. Wu, Anal. Chim. Acta 967 (2017) 5258.

  • [13]

    H.S. Parmar , P. Jain, D.S. Chauhan, M.K. Bhinchar, V. Munjal, M. Yusuf, K. Choube, A. Tawani, V. Tiwari, E. Manivannan, A. Kumar, Diabetes Res. Clin. Pract. 97 (2012) 105111.

    • Search Google Scholar
    • Export Citation

For Submission Guideline click HERE

Manuscript Submission: JPC Manuscript Submission

Senior editors

Editor(s)-in-Chief: B. Spangenberg

Managing Editor: Kakuk Ágnes

Editorial Board

  • T.H. Dzido (Lublin, Poland)
  • Á. M. Móricz (Budapest, Hungary)
  • C.F. Poole (Detroit, MI, USA)
  • E. Reich (Muttenz, Switzerland)
  • B. Renger (Radolfzell, Germany)
  • I. Vovk (Ljubljana, Slovenia)

 

Advisory Board

  • E. BODOKI (Cluj-Napoca, Romania)
  • U. A. TH. BRINKMAN (Amsterdam, The Netherlands)
  • I. CHOMA (Lublin, Poland)
  • V. COMAN (Cluj-Napoca, Romania)
  • W. DAMMERTZ (Kressbronn, Germany)
  • K. FERENCZI-FODOR (Budapest, Hungary)
  • G. INDRAYANTO (Surabaya, Indonesia)
  • R. E. KAISER (Bad Dürkheim, Germany)
  • I. KLEBOVICH (Budapest, Hungary)
  • T. KOWALSKA (Katowice, Poland)
  • L. LEPRI (Florence, Italy)
  • E. MINCSOVICS (Budapest, Hungary)
  • D. NUROK (Indianapolis, IN, USA)
  • B. RENGER (Radolfzell, Germany)
  • J. K. RÓŻYŁO (Lublin, Poland)
  • J. SHERMA (Easton, PA, USA)
  • M. WAKSMUNDZKA-HAJNOS (Lublin, Poland)
  • D. WEDGE (Mississippi, USA)

A. Kakuk - Managing Editor, Akadémiai Kiadó
Address: P.O. Box 245. H-1519 Budapest, Hungary
Fax: +36 1 464 82 95
E-mail: jpc@akkrt.hu

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences

 

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2021 5 0 0
Jul 2021 6 0 0
Aug 2021 7 0 0
Sep 2021 3 1 2
Oct 2021 7 0 0
Nov 2021 3 0 0
Dec 2021 0 0 0