Coupling of a sheep anti-T3 IgG to a solid phase for a triiodothyronine radioimmunoassay

I. Santos; F. Waerenborgh; L. Patricio

doi:10.1007/bf02037955

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 102: Issue 1

Coupling of a sheep anti-T₃ IgG to a solid phase for a triiodothyronine radioimmunoassay

Authors:

I. Santos

I. Santos Laboratório Nacional Engineering Technologia Industrial Sector de Radioisótopos Estrada Nacional, 10 2686 Sacavém Codex Portugal

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F. Waerenborgh

F. Waerenborgh Laboratório Nacional Engineering Technologia Industrial Sector de Radioisótopos Estrada Nacional, 10 2686 Sacavém Codex Portugal

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L. Patricio

L. Patricio Laboratório Nacional Engineering Technologia Industrial Sector de Radioisótopos Estrada Nacional, 10 2686 Sacavém Codex Portugal

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Pages:: 143–148

Online Publication Date:: 13 Aug 2005

Publication Date:: 17 Nov 1986

Article Category:: Research Article

DOI:: https://doi.org/10.1007/bf02037955

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Abstract

Optimal conditions for coupling sheep anti-T₃ IgG to a solid phase are presented. We found that the optimal activation of microcrystalline cellulose was achieved with 0.15M 1,1-carbonyldiimidazol (CDI) in acetone. We also found that using a 25 mg/cm³ anti-T₃ IgG solution, in barbitone buffer 0.05M, pH 8.0, we could get a reasonable yield of coupling and a remaining solution of anti-T₃ IgG (first supernatant) with a suitable concentration (10 mg/cm³) for another coupling. The solid phase anti-T₃ obtained in these two couplings present similar characteristics which make possible their use in a total T₃ RIA.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)