A rapid neutron activation method was developed to determine very low levels of uranium (gl–1) in groundwater from the Palmyrides region, Central Syria. The239U
-ray at 74.7 keV energy was used in the measurements. Results of this technique were compared against the conventional neutron activation analysis (INAA) with239Np and the laser-induced fluoremetry (LIF). In general, the three data sets showed a very good agreement. The described method provides good accuracy, precision and low detection limit, particularly when the total dissolved solids (TDS) of the samples is low.