A simplified radioimmunoassay for triiodothyronine (T3) using pre-incubated labelled antigen and antibody

M. Pillai; U. Nagvekar; C. Desai; R. Mani

doi:10.1007/bf02047918

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 102: Issue 2

A simplified radioimmunoassay for triiodothyronine (T₃) using pre-incubated labelled antigen and antibody

Authors:

M. Pillai

M. Pillai ISOPHARM Radiopharmaceuticals Division, Bhabha Atomic Research Centre Turbhe Complex 400 705 New Bombay India

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U. Nagvekar

U. Nagvekar ISOPHARM Radiopharmaceuticals Division, Bhabha Atomic Research Centre Turbhe Complex 400 705 New Bombay India

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C. Desai

C. Desai ISOPHARM Radiopharmaceuticals Division, Bhabha Atomic Research Centre Turbhe Complex 400 705 New Bombay India

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, and

R. Mani

R. Mani ISOPHARM Radiopharmaceuticals Division, Bhabha Atomic Research Centre Turbhe Complex 400 705 New Bombay India

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Pages:: 435–441

Online Publication Date:: 15 Aug 2005

Publication Date:: 17 Dec 1986

Article Category:: Research Article

DOI:: https://doi.org/10.1007/bf02047918

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Abstract

We discribe the development of a simplified radioimmunoassay for triiodothyronine (T₃) using pre-incubated labelled T₃ and antibody. The assay is carried out by adding 50 l of standard or sample to 0.4 ml of pre-incubated reagent dispensed in assay tubes. The reaction is allowed to proceed for about four hours and the antigen-antibody complex precipitated by the addition of 1 cm³ of 22% polyethylene glycol solution. Due to the high dissociation constant of T₃-antibody complex at 37° C (2.83·10^–4 S^–1), the labelled antigen-antibody complex dissociates and thereby the unlabelled antigen binds with the antibody. With a four hour incubation the sensitivity of this assay is comparable to an assay done by the equilibrium method using the same antibody. Sixty serum samples were analyzed using this method and compared with the equilibrium assay (Y=0.94x+0.046 ng/cm³, r=0.98).

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)