Radioimmunoassay of Human Follicle Stimulating Hormone /HFSH/

M. Pimpalkhute; M. Majali; R. Mani

doi:10.1007/bf02162766

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 103: Issue 2

Radioimmunoassay of Human Follicle Stimulating Hormone /HFSH/

Authors:

M. Pimpalkhute

M. Pimpalkhute Bhabha Atomic Research Centre Radiopharmaceuticals Division Trombay 400 085 Bombay India

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M. Majali

M. Majali Bhabha Atomic Research Centre Radiopharmaceuticals Division Trombay 400 085 Bombay India

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, and

R. Mani

R. Mani Bhabha Atomic Research Centre Radiopharmaceuticals Division Trombay 400 085 Bombay India

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Pages:: 105–116

Online Publication Date:: 27 Sep 2005

Publication Date:: 17 Jan 1986

Article Category:: Research Article

DOI:: https://doi.org/10.1007/bf02162766

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Abstract

A method for determining Human FSH in blood/serum by RIA is described. The radioiodination of FSH with¹²⁵I is carried out under carefully controlled conditions such as, amount of initial activity of¹²⁵I take for iodination, the reaction volume, and the reaction time, etc. The tracer FSH obtained thus is with minimum damage and optimum specific activity which is ideally suited for RIA. The shelf-life of the tracer is enhanced by the addition of benzyl alchol. The tracer can be conveniently stored at+4–6°C upto 10 weeks, avoiding the repeated freezing and thawing process. Antiserum to FSH is raised in rabbits by repeated injections via intramuscular route. The method utilizes polyethylene glycol /PEG/ as the separation system. Using this method, a number of control samples of men and women of reproductive age group are screened. This sensitive assay has a good validity and has an inter-assay variation less than 15%.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)