Preparation and characterization of reagents for epitestosterone radioimmunoassay

R. Bilek; R. Hampl; Z. Putz; L. Stárka

doi:10.1007/bf02164702

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 108: Issue 3

Preparation and characterization of reagents for epitestosterone radioimmunoassay

Authors:

R. Bilek

R. Bilek Research Institute of Endocrinology 116 94 Prague 1 Czechoslovakia

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R. Hampl

R. Hampl Research Institute of Endocrinology 116 94 Prague 1 Czechoslovakia

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Z. Putz

Z. Putz Institute of Clinical Endocrinology 034 91 Lubochňa Czechoslovakia

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L. Stárka

L. Stárka Research Institute of Endocrinology 116 94 Prague 1 Czechoslovakia

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Pages:: 159–168

Online Publication Date:: 27 Sep 2005

Publication Date:: 17 Feb 1987

Article Category:: Research Article

DOI:: https://doi.org/10.1007/bf02164702

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Abstract

The preparation of reagents, suitable for radioimmunoassay of 17-hydroxy-4-androsten-3-one /epitestosterone/ in body fluids is described. It includes a simplified synthesis of the immunogen, epitestosterone-3-/O-carboxymethyl/oxime coupled to bovine serumalbumin, its characterization and preparation of the radioligand [¹²⁵I] iodohistaminyl derivative of epitestosterone. The assay protocol for epitestosterone detection in urine avoiding extraction is described and the reliability criteria of the method are given.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)