Authors:
A. Glagoličová Charles University Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty Prague (ČSFR)

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V. Wernischová Charles University Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty Prague (ČSFR)

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F. Novák Charles University Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty Prague (ČSFR)

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Abstract  

For purposes of radioimmunoanalytical determination of serum ferritin, conditions for antigen iodination and separation were searched for, which could provide a satisfactory radiochemical purity and specific activity, high immunoreactivity and stability of the resulting labeled product, necessary for an acceptable expiration of the RIA kit. Two iodination methods (chloramine and conjugation methods) were tested, and a three-step procedure was elaborated for iodination and separation by gel column chromatography. The iodinated antigen obtained —125I-placental ferritin with IRmax of about 80%,125I<8%, specific activity of about 0.6MBq/g and stability for the expiration period of 3 to 4 months — is quite satisfactory for the RIA applications.

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)