Journal of Radioanalytical and Nuclear Chemistry
Volume/Issue:
Volume 177: Issue 2
Preparation and characterization of125I labeled progesterone derivatives for the development of a radioimmunoassay for progesterone
Authors:
K. Kothari Bhabha Atomic Research Centre Isotope Division 400 085 Bombay (India)

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M. Pillai Bhabha Atomic Research Centre Isotope Division 400 085 Bombay (India)

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Pages:
261–269
Online Publication Date:
14 Aug 2005
Publication Date:
17 Jan 1994
Article Category:
Research Article
DOI:
https://doi.org/10.1007/bf02061122
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Abstract  

Preparation and purification of radioiodinated progesterone derivatives for the development of a radioimmunoassay of progesterone is described. We have standardized two procedures for preparing radioiodinated progesterone conjugate. In the first procedure125I labeled histamine was conjugated to progesterone 11 hemisuccinate by the mixed anhydride method. In the secod procedure, tyrosyl methyl ester was conjugated to progesterone 11 hemisuccinate and iodination of the conjugate was carried out. Purification of the iodinated products was carried out by solvent extraction and thin layer chromatography techniques. The radiochemical purity of the tracers prepared by both methods were more than 95%. Labeled progesterone derivatives prepared were used for developing a radioimmunoassay procedure. The non-specific binding of the tracer was about 2–3%, while up to 80% binding could be obtained in the presence of excess antibody. The radioiodinated tracer could be used up to four months in the assay.

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Cover Journal of Radioanalytical and Nuclear Chemistry
Journal of Radioanalytical and Nuclear Chemistry
Print ISSN:
0236-5731
Online ISSN:
1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation		 1968
Volumes
per Year		 1
Issues
per Year		 12
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)

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