Author:
S. Javaid Khurshid Pakistan Institute of Nuclear Science and Technology Nuclear Chemistry Division P. O. Nilore Islamabad Pakistan

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Abstract  

Estimation of vitamin B12 in blood is very important to determine in decifiency and diagnosis of anemic patients. Vitamin B12 in blood can be estimated by spectrochemical, enzymatic, radioisotopic and microbiological methods. In the present study vitamin B12 was determined in 48 normal subjects of Rawalpindi/Islamabad by radiometric microbiological assay (RMA) technique using a very rapid, sensitive and automated instrument Bactec 460. In this procedure14C-glucose media and microorganismsLactobacillus leichmannii were used. The sensitivity of the method for vitamin B12 is 1 pg/ml and the vitamin B12 found in normal subjects was in the range of 105–535 pg/ml with a median value of 246±6 pg/ml.

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)