Journal of Radioanalytical and Nuclear Chemistry
Volume/Issue:
Volume 185: Issue 1
Radiometric microbiological estimation of vitamin B12
Author:
S. Javaid Khurshid Pakistan Institute of Nuclear Science and Technology Nuclear Chemistry Division P. O. Nilore Islamabad Pakistan

Search for other papers by S. Javaid Khurshid in
Current site
Google Scholar
PubMed Close
Pages:
167–171
Online Publication Date:
13 Aug 2005
Publication Date:
17 Nov 1994
Article Category:
Research Article
DOI:
https://doi.org/10.1007/bf02042963
Restricted access

Abstract  

Estimation of vitamin B12 in blood is very important to determine in decifiency and diagnosis of anemic patients. Vitamin B12 in blood can be estimated by spectrochemical, enzymatic, radioisotopic and microbiological methods. In the present study vitamin B12 was determined in 48 normal subjects of Rawalpindi/Islamabad by radiometric microbiological assay (RMA) technique using a very rapid, sensitive and automated instrument Bactec 460. In this procedure14C-glucose media and microorganismsLactobacillus leichmannii were used. The sensitivity of the method for vitamin B12 is 1 pg/ml and the vitamin B12 found in normal subjects was in the range of 105–535 pg/ml with a median value of 246±6 pg/ml.

  • Collapse
  • Expand
Cover Journal of Radioanalytical and Nuclear Chemistry
Journal of Radioanalytical and Nuclear Chemistry
Print ISSN:
0236-5731
Online ISSN:
1588-2780

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation		 1968
Volumes
per Year		 1
Issues
per Year		 12
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 2 0 0
Dec 2024 6 0 0
Jan 2025 2 0 0
Feb 2025 4 0 0
Mar 2025 6 0 0
Apr 2025 9 0 0
May 2025 0 0 0