Journal of Radioanalytical and Nuclear Chemistry
Volume/Issue:
Volume 206: Issue 1
Development of RAST assay for determination of anti- Populus canadensis IgE antibodies
Authors:
T. Trtić Vinca Institute of Nuclear Sciences P. O. Box 502 Belgrade (Yugoslavia)

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L. Vuksanović Vinca Institute of Nuclear Sciences P. O. Box 502 Belgrade (Yugoslavia)

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S. Paranos University Hospital “Zvezdara” Belgrade (Yugoslavia)

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S. Petrović Torlak Institute of Immunobiology and Virology Belgrade (Yugoslavia)

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Z. Vujčić University of Belgrade Faculty of Chemistry Belgrade (Yugoslavia)

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I. Plećas Vinca Institute of Nuclear Sciences P. O. Box 502 Belgrade (Yugoslavia)

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R. Jankov University of Belgrade Faculty of Chemistry Belgrade (Yugoslavia)

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Pages:
145–149
Online Publication Date:
13 Aug 2005
Publication Date:
17 May 1996
Article Category:
Research Article
DOI:
https://doi.org/10.1007/bf02040051
Restricted access

Abstract  

One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb E1 was used for forming assay for the determination of specific IgE. By titration of extract of poplarPopulus canadensis we determined that the quantity of 0.65 mL extract is needed for coupling of lg BrCN activated paper discs. Coupling was performed in Na2CO3/NaHCO3 buffer pH 10, on 4°C for 48h. Using this newely formed RAST, specific forPopulus canadensis, we have deteminated anti-Populus canadensis IgE antibodies as well as cross reactivity between pollens ofPopulus canadensis andPopulus deltoides.

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Cover Journal of Radioanalytical and Nuclear Chemistry
Journal of Radioanalytical and Nuclear Chemistry
Print ISSN:
0236-5731
Online ISSN:
1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation		 1968
Volumes
per Year		 1
Issues
per Year		 12
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)

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