Journal of Radioanalytical and Nuclear Chemistry
Volume/Issue:
Volume 206: Issue 1
Direct labelling of anti-gastric cancer monoclonal antibody 3H11 with99mTc
Authors:
Yang Zhi Beijing Medical University Beijing Institute for Cancer Research and School of Clinical Oncology 100034 Beijing (P.R. China)

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Zhang Meiying Beijing Medical University Beijing Institute for Cancer Research and School of Clinical Oncology 100034 Beijing (P.R. China)

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Lin Baohe Beijing Medical University Beijing Institute for Cancer Research and School of Clinical Oncology 100034 Beijing (P.R. China)

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Han Yan Beijing Medical University Beijing Institute for Cancer Research and School of Clinical Oncology 100034 Beijing (P.R. China)

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Mu Aping Beijing Medical University Beijing Institute for Cancer Research and School of Clinical Oncology 100034 Beijing (P.R. China)

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Zhang Qing Beijing Medical University Beijing Institute for Cancer Research and School of Clinical Oncology 100034 Beijing (P.R. China)

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Xu Xiaobao Beijing Medical University Beijing Institute for Cancer Research and School of Clinical Oncology 100034 Beijing (P.R. China)

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Pages:
59–67
Online Publication Date:
13 Aug 2005
Publication Date:
17 May 1996
Article Category:
Research Article
DOI:
https://doi.org/10.1007/bf02040042
Restricted access

Abstract  

Monoclonal antibody 3H11 was labelled with99mTc by modified Schwartz method. The antibody was incubated in a glass test tube at room temperature for 30min with a 1000-fold molar excess of 2-mercaptoethanol. The mean labelling efficiency of 3H11 with99mTc was more than 95%. The immuonreactivity of99mTc-3H11 was more than 80% by ELISA's method. Competetion results in vitro and HPLC analysis showed that99mTc was combined at the high affinity sites of antibody. The biodistribution in nude mice bearing 823 gastric cancer xenograpfts showed that the radioactivity in tumor at 24h postinjection was the highest except for that in kidney. The tumor uptake was 8.98±2.42% i.d/g. The ratio of tumor to blood was over 1.5 and that of tumor to liver was more than 2.5 at 24h post-injection. The tumor was clearly imaged at 22h postinjection. The inital clinical results showed that99mTc-3H11 was stable in vivo and was good located at the lesion sites.

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Cover Journal of Radioanalytical and Nuclear Chemistry
Journal of Radioanalytical and Nuclear Chemistry
Print ISSN:
0236-5731
Online ISSN:
1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation		 1968
Volumes
per Year		 1
Issues
per Year		 12
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)

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