Kit development for labelling erythrocytes or leukocytes with technetium-99m in vitro

Wang Xuebin; Han Quansheng; Wang Jingcheng; Wang Qian; Mi Hongzhi

doi:10.1007/bf02040043

Jump to Content

Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 206: Issue 1

Kit development for labelling erythrocytes or leukocytes with technetium-99m in vitro

Authors:

Wang Xuebin

Wang Xuebin Beijing Normal University Department of Chemistry 100875 Beijing (P.R. China)

Search for other papers by Wang Xuebin in
Current site
Google Scholar
PubMed

Han Quansheng

Han Quansheng Beijing Normal University Department of Chemistry 100875 Beijing (P.R. China)

Search for other papers by Han Quansheng in
Current site
Google Scholar
PubMed

Wang Jingcheng

Wang Jingcheng Beijing Anzhen Hospital Department of Nuclear Medicine 100029 Beijing (P.R. China)

Search for other papers by Wang Jingcheng in
Current site
Google Scholar
PubMed

Wang Qian

Wang Qian Beijing Normal University Department of Chemistry 100875 Beijing (P.R. China)

Search for other papers by Wang Qian in
Current site
Google Scholar
PubMed

, and

Mi Hongzhi

Mi Hongzhi Beijing Anzhen Hospital Department of Nuclear Medicine 100029 Beijing (P.R. China)

Search for other papers by Mi Hongzhi in
Current site
Google Scholar
PubMed

View More View Less

Pages:: 69–76

Online Publication Date:: 13 Aug 2005

Publication Date:: 17 May 1996

Article Category:: Research Article

DOI:: https://doi.org/10.1007/bf02040043

Restricted access

Abstract

This study developed a modified pretinning method for labeling erythrocytes with technetium-99m in vitro using a kit which contains stannous chloride stabilized with gentisic acid (GA) in lyophilized form. This GA-kit is a sterile closed system that does not require centrifugation, washing and separation of the RBCs. The kit is simple to use and labeling takes 30 min with a typical labeling efficiency of 97.3%±1.2% for 60 determinations. Imaging studies were performed in rabbit using GA-kit in vitro labeling procedure and PYP-kit in vivo labeling. It shows that the heart-to-background ratio with GA in vitro labeling were significantly higher than with in vivo labeling. Leukocytes labeling with technetium-99m using the same GA kit also was studied. The labeling efficiency was 20–41%. Thus, the GA kit is versatile and cost-effective.

Save
Cite
Email this content

Share Link

Copy this link, or click below to email it to a friend
Email this content
or copy the link directly:

https://akjournals.com/abstract/journals/10967/206/1/article-p69.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)