A method for preparation of magnetizable cellulose and its application in radioimmunoassays for T3 and T 41

V. Kadwad; N. Jyotsna; N. Sivaprasad; P. Sinha

doi:10.1007/bf02055403

Jump to Content

Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 210: Issue 1

A method for preparation of magnetizable cellulose and its application in radioimmunoassays for T₃ and T ₄¹

Authors:

V. Kadwad

V. Kadwad Board of Radiation and Isotope Technology (BRIT) Radiopharmaceuticals and Labeled Compounds V.N. Purav Marg, Deonar 400 094 Bombay India

Search for other papers by V. Kadwad in
Current site
Google Scholar
PubMed

N. Jyotsna

N. Jyotsna Board of Radiation and Isotope Technology (BRIT) Radiopharmaceuticals and Labeled Compounds V.N. Purav Marg, Deonar 400 094 Bombay India

Search for other papers by N. Jyotsna in
Current site
Google Scholar
PubMed

N. Sivaprasad

N. Sivaprasad Board of Radiation and Isotope Technology (BRIT) Radiopharmaceuticals and Labeled Compounds V.N. Purav Marg, Deonar 400 094 Bombay India

Search for other papers by N. Sivaprasad in
Current site
Google Scholar
PubMed

, and

P. Sinha

P. Sinha BARC Vashi Complex Powder Metallurgy Division, Bhabha Atomic Research Centre 400 705 New Bombay India

Search for other papers by P. Sinha in
Current site
Google Scholar
PubMed

View More View Less

Pages:: 27–33

Online Publication Date:: 13 Aug 2005

Publication Date:: 17 Oct 1996

Article Category:: Research Article

DOI:: https://doi.org/10.1007/bf02055403

Restricted access

Abstract

An easy adoptable technique has been developed to prepare magnetizable cellulose by the process of wet grinding of a mixture of iron oxide (magnetite) and cellulose in a stirred ball mill. Anti rabbit IgG was covalently coupled to this magnetizable cellulose. The immunosorbent thus prepared has been used in radioimmunoassays of T₃ and T₄ for the separation of bound and free antigens. The reliability of the above immunosorbent has been validated by studying assay parameters such as non-specific binding (NSB), maximum binding (B₀), CV etc. The single step process of wet grinding adopted here not only firmly binds magnetite and cellulose physically, but also lowers the size of the resulting magnetizable cellulose. The conventional method of preparation involves the coprecipitation of cellulose dissolved in cuprammonium hydroxide solution along with iron oxide, followed by extensive grinding and sieving of the iron oxide impregnated cellulose.

Save
Cite
Email this content

Share Link

Copy this link, or click below to email it to a friend
Email this content
or copy the link directly:

https://akjournals.com/abstract/journals/10967/210/1/article-p27.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)