The possibilities of preparing a packing for reversed phase column chromatography from Menderes' perlite were studied. Its
physical and chemical characteristics were compared with other solid supports prepared from rocks, such as perlite and volcanic
slags. A series of chemical treatments were applied to improve the mechanical and chemical properties of perlite. The experimental
work covers the strong acid treatment, the strong base treatment and the silanization with DMCS. The raw perlite containing
70–73% SiO2 was treated with NaOH to make soluble silicates and to increase it. Thus the surface and mechanical characters of the modified
perlite were determined. The mean surface OH group density and the specific surface area were 3.2 μmol/m2 and 9.2 m2/g, respectively. The modified perlite was silanized and hydrophized to load organic complexing agents. The TBP was fixed
successfully on perlite up to 20% w/w. The packing prepared was used to study the chromatographic behavior of UO22+ Fe3+.