Lipiodol has excellent retainable ability in hepatoma cells. This agent can be labeled with radioisotope (131I) and mixed with tissue adhesive (Histoacryl), and then alttached on the lesion of liver by intrahepatic arterial administration. In this study, we attempt to obtain the optimal ratio of Lipiodol to Histoacryl and evaluate the consolidation of blood in vitro and toxicity and biodistribution in vivo. The ratio of131I Lipiodol/Histoacryl mixture (L/H), concentration of heparin and flow rate of blood are varied by simulating the installation of bloodstream to test the time of consolidation. In addition, the optimal ratios of the L/H mixtures are assessed in vitro in heparinized human blood. According to those results, Lipiodol and Histoacryl mixed with 1∶1 or 2∶1 ratio have an ideal time of 13 to 15 seconds in vitro; in addition, 1.2∶1 ratio is an optimal ratio in the biodistribution study. Interestingly, heparin and acetic acid does not alter the consolidation time, in addition, no variation occurs when varying the flow rate of blood. The consolidation of L/H mixture with blood is incubated in the 37°C, normal saline bath for 24 hours. No dissociation of free131I is found. The optimal mixture is also injected into the hepatic artery of the Sprague-Dawley rats carrying for 24 hours. No dissociation of free131I is found. The optimal mixture is also injected into the hepatic artery of the Sprague-Dawley rats carrying hepatocellular carcinoma (NIS1 cell line). Radioactive consolidate is well confined in the tumor without evidence of leakage of the mixture to the lung or distribution of free131I in the thyroid. In conclusion, this mixture has the merits of both irradiation and embolization of the tumor. The131I Lipiodol/Histoacryl mixture (1.2∶1) is a promising alternative for intrahepatic arterial administration to treat hepatic tumors. Histoacryl can confine the131I and, also, embolize the tumor vessels.