Prompt gamma-rays from thermal neutron capture reaction have been used to measure the concentrations of the main constituents namely Fe, Ni and Cr in 316-L stainless steel using recently established prompt gamma neutron activation analysis (PGNAA) facility at Pakistan Institute of Nuclear Science and Technology (PINSTECH). High resolution, high purity germanium detector with 40% relative efficiency was employed for the gamma-ray spectroscopy of the samples. The interference-free full energy gamma-ray peaks of the elements of interests were selected in the high energy low background region (5.0–9.0 MeV). The efficiency calibration of the detector was performed using ultra pure standards of chromium and chlorine obtained respectively from Merck and Alpha Inorganics. This paper describes, in addition, the salient features as well as the background of establishing PGNAA facility at the Institute.