Chemical isotope effects of potassium and rubidium were studied bythe liquid-liquid extraction using a crown ether of dicyclohexano-18-crown-6.The isotope enrichment factors for unit mass difference were 0.0062+0.0013for potassium and 0.0018+0.0005 for rubidium. The correlation between theobtained isotope enrichment factors and the atomic weights was discussed withthe applicable values of literatures for the alkali metals.