Determination of metal-binding proteins in soft tissues of rock oyster by neutron activation analysis and particle induced X-ray emission

M. Fukushima; H. Tamate; N. Fujii; Y. Nakano

doi:10.1023/a:1024701527789

Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 257: Issue 1

Determination of metal-binding proteins in soft tissues of rock oyster by neutron activation analysis and particle induced X-ray emission

Authors:

M. Fukushima

M. Fukushima Ishinomaki Senshu University Department of Sciences and Engineering Ishinomaki, Miyagi 986-8580 Japan

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H. Tamate

H. Tamate Ishinomaki Senshu University Department of Sciences and Engineering Ishinomaki, Miyagi 986-8580 Japan

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N. Fujii

N. Fujii Kyoto University Research Reactor Institute Noda, Kumatori, Sennnan, Osaka 590-0451 Japan

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Y. Nakano

Y. Nakano Kyoto University Research Reactor Institute Noda, Kumatori, Sennnan, Osaka 590-0451 Japan

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Pages:: 105–108

Online Publication Date:: 31 Oct 2004

Publication Date:: 17 Jul 2003

Article Category:: Research Article

DOI:: https://doi.org/10.1023/a:1024701527789

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Abstract

In this work, we determined the levels of trace metals in protein fractions isolated from rock oysters by neutron activation analysis (NAA) and particle induced X-ray emission (PIXE). Proteins were extracted from mantles and hepatopancreases of rock oysters and fractionated by size-exclusion high performance liquid chromatography (HPLC). The protein fractions from mantles and hepatopancreases are found to be abundant in Fe, Cu, Zn, Mn, Pb, and Ag. HPLC profiles of Fe, Cu, Zn, and Ag indicate that those elements are bound to proteins extracted from mantles and hepatopancreases.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)