A Ni-Cr alloy was chosen as an in-house reference material for neutron flux measurement due to the well-known physical constants of the nuclides or interest and its low cost. The quantification of the Cr and Ni content was performed by neutron activation analysis (NAA), using certified reference materials from NIST as comparators. The concertrations and standard combined uncertainties were 19.32 ± 0.07 % for Cr and 80.2 ± 1.1 % for Ni, and the material is very homogeneous for Cr and moderate homogeneous for Ni.