Determination of selenium in red blood cells by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion

U. Tinggi; T. Gianduzzo; R. Francis; D. Nicol; M. Shahin; P. Scheelings

doi:10.1023/b:jrnc.0000020920.28409.30

Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 259: Issue 3

Determination of selenium in red blood cells by inductively coupled plasma mass spectrometry (ICP-MS) after microwave digestion

Authors:

U. Tinggi

U. Tinggi Queensland Health Scientific Services Centre for Public Health Sciences 39 Kessels Road Coopers Plains Queensland 4108 Australia

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T. Gianduzzo

T. Gianduzzo Princess Alexandra Hospital Department of Surgery Ipswich Road Woolloongabba Queensland 4102 Australia

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R. Francis

R. Francis Queensland Health Scientific Services Centre for Public Health Sciences 39 Kessels Road Coopers Plains Queensland 4108 Australia

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D. Nicol

D. Nicol Princess Alexandra Hospital Department of Surgery Ipswich Road Woolloongabba Queensland 4102 Australia

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M. Shahin

M. Shahin Princess Alexandra Hospital Department of Surgery Ipswich Road Woolloongabba Queensland 4102 Australia

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P. Scheelings

P. Scheelings Queensland Health Scientific Services Centre for Public Health Sciences 39 Kessels Road Coopers Plains Queensland 4108 Australia

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Pages:: 469–472

Online Publication Date:: 16 Aug 2006

Publication Date:: 01 Mar 2004

Article Category:: Research Article

DOI:: https://doi.org/10.1023/b:jrnc.0000020920.28409.30

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Abstract

Selenium is an essential trace element and its levels in blood have been widely used for assessing Se status in humans. The aim of this present study is to develop a suitable method for the determination of Se in red blood cells (RBC) using ICP-MS after microwave digestion. The blood samples were obtained from patients with benign prostatic hyperplasia (BPH), who attended urology clinics at the Princess Alexandra hospital, Brisbane, Australia. No apparent polyatomic and matrix interferences were encountered when ⁸²Se isotope was used for the analysis of Se levels in RBC. Whole Blood Seronorm Trace Elements (SERO, Norway) and dogfish muscle (DORM-1, NRCC) were used as reference materials for method validation. The method was rapid and accurate, and ideal for routine analysis of Se in RBC, and in particular for assessing of Se status in humans.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)