For the analysis of metal-containing proteins, sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been
synchrotron radiation X-ray fluorescence (SRXRF). In a pilot study the applicability of this combined method was tested in
the analysis of metalloaded
apoazurin and of the selenoproteins in rat testis homogenate. It was shown that it can be applied in the determination of
the major stable
binding forms of trace elements. After further improvement of the limits of detection the method will allow the analysis of
proteins present in the samples at low concentrations.