The formation of plutonium radionuclides (239+240Pu) from uranium was determined in dismounted shielding concrete from accelerator components. Plutonium and uranium fractions were separated by radioanalytical techniques and measured by -spectroscopy. The measurements are consistent with yield calculations based on transport and single particle codes. The yield of 239+240Pu did not exceed the two-fold exemption limit given in the Swiss Radiation Protection Law, thus the plutonium content in shielding concrete should not cause problem for the environment.