Labeling of the anti-melanoma 14f7 monoclonal antibody with rhenium-188-MAG3 chelate: Conjugation optimization, in vitro stability and animal studies

J. Crudo; M. Edreira; E. Obenaus; S. de Castiglia

doi:10.1023/b:jrnc.0000034868.70027.a1

Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 261: Issue 2

Labeling of the anti-melanoma 14f7 monoclonal antibody with rhenium-188-MAG₃ chelate: Conjugation optimization, in vitro stability and animal studies

Authors:

J. Crudo

J. Crudo Comisión Nacional de Energía Atómica, Centro Atómico Ezeiza División Radiofármacos Pbro. Gonzales y Aragon Num. 15, (B1802AYA) Ezeiza, Buenos Aires Argentina

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M. Edreira

M. Edreira Comisión Nacional de Energía Atómica, Centro Atómico Ezeiza División Radiofármacos Pbro. Gonzales y Aragon Num. 15, (B1802AYA) Ezeiza, Buenos Aires Argentina

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E. Obenaus

E. Obenaus Comisión Nacional de Energía Atómica, Centro Atómico Ezeiza División Radiofármacos Pbro. Gonzales y Aragon Num. 15, (B1802AYA) Ezeiza, Buenos Aires Argentina

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, and

S. de Castiglia

S. de Castiglia Comisión Nacional de Energía Atómica, Centro Atómico Ezeiza División Radiofármacos Pbro. Gonzales y Aragon Num. 15, (B1802AYA) Ezeiza, Buenos Aires Argentina

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Pages:: 337–342

Online Publication Date:: 03 Nov 2004

Publication Date:: 17 Aug 2004

Article Category:: Research Article

DOI:: https://doi.org/10.1023/b:jrnc.0000034868.70027.a1

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Abstract

Rhenium-188 is a high energy -emitter that has a therapeutic promise. The aim of the present study was to label the anti-melanoma 14f7 monoclonal antibody (MoAb) with ¹⁸⁸Re, using an active ester of S-benzoyl-mercaptoacetyltriglycine (S-benzoyl-MAG₃) and to evaluate its tumor uptake in C57 mice bearing a B16F0 melanoma. The optimum labeling efficiency was 49.6% and the maximal tumor uptake was 8.8%ID/g at 24 hours. Quality assurance tests were performed during the entire labeling procedure and on the final radiolabeled MoAb. Stability studies in saline and human serum were also performed.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)