Changes in the secondary structure of proteins labeled with 125I: CD spectroscopy and enzymatic activity studies

Y. M. Efimova; B. Wierczinski; S. Haemers; A. A. van Well

doi:10.1007/s10967-005-0679-8

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 264: Issue 1

Changes in the secondary structure of proteins labeled with ¹²⁵I: CD spectroscopy and enzymatic activity studies

Authors:

Y. M. Efimova

Y. M. Efimova Department of Radiochemistry, Delft University of Technology Mekelweg 15, 2629 JB Delft, The Netherlands

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B. Wierczinski

B. Wierczinski Department of Radiochemistry, Delft University of Technology Mekelweg 15, 2629 JB Delft, The Netherlands

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S. Haemers

S. Haemers Department of Radiochemistry, Delft University of Technology Mekelweg 15, 2629 JB Delft, The Netherlands

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, and

A. A. van Well

A. A. van Well Department of Neutron Scattering and Mössbauer Spectroscopy, Interfaculty Reactor Institute, Delft University of Technology Mekelweg 15, 2629 JB Delft, The Netherlands

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Pages:: 91–96

Online Publication Date:: 24 Mar 2005

Publication Date:: 17 Mar 2005

Article Category:: Research Article

DOI:: https://doi.org/10.1007/s10967-005-0679-8

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Summary

Bovine serum albumin (BSA) and lysozyme (LSZ) were radiolabeled with¹²⁵I. Three different methods for protein iodination with ¹²⁵I were optimized. Parameters like incubation time and ratio of oxidizing agent and amount of protein were established. During protein iodination with ¹²⁵I, structural damages caused by the introduction of iodine into the protein may occur. These damages depend on the oxidizing agent used and may lead to considerable changes in the protein structure and, hence, their biological activity. Changes in secondary structure of LSZ and BSA were examined by circular dichroism (CD). Enzymatic activity tests were performed with lysozyme to check its biological activity. The Iodo Bead was found the best oxidizing agent for protein iodination.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)