The objective of this study was to investigate the bacterial adherence to a non-precious alloy with radiolabeling method.
S. mutans, E. coliand C. albicanswere labeled with 99mTc by using stannous chloride and their radiolabeling yields were calculated. After the labeling procedure, metal disks (3
mm×10 mm) were treated with microorganisms. The amount of labeled microorganisms adhered on metal surfaces was determined
by activity measurements. The labeling yields for S. mutans, E. coliand C. albicanswere 69.95±7.58%, 78.84±0.44% and 79.71±10.17%, respectively. The mean values for adherence for S. mutans, E. coliand C. albicanson metal samples were 7.02±2.18%, 0.96±0.49% and 8.80±8.24%, respectively. The radiolabeling method could be considered as
safe and precise for determining the adherence of microorganisms.