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  • 1 Jockey Club Brasileiro Av. Bartolomeu Mitre 1314, 22431-000, Rio de Janeiro, Brazil
  • | 2 Pontifícia Universidade Católica Rua Marqu?s de S?o Vicente 225, 22453-900, Rio de Janeiro, Brazil
  • | 3 Centro de Energia Nuclear na Agricultura, University of S?o Paulo 13400-970, PO Box 96, Piracicaba, SP, Brazil
  • | 4 Delft University of Technology, Reactor Institute Delft Mekelweg 15, 2629JB, Delft, The Netherlands
  • | 5 Centro de Energia Nuclear na Agricultura, University of S?o Paulo 13400-970, PO Box 96, Piracicaba, SP, Brazil
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Summary  

This paper describes an analytical method using a nuclear-related technique for the detection of forbidden doping substances in the urine of race horses. The proposed method, adapted from the Méthode Alcaline Sur C-18 developed by the French Laboratoire de Contrôle Antidopage, is based on gas chromatography separation followed by mass spectrometry (GC-MS). The method was validated for caffeine, identified as the most frequent doping substance in the Brazilian horseracing activity. This validation is also a major requirement to achieve ISO/IEC 17025 laboratory accreditation. The validation has led to several metrological challenges because the decisions are largely based on qualitative results (“false-positive” and/or “false-negative”) and the degree of accuracy, as well as the traceability had to be determined in the absence of certified matrix reference materials.