Authors:
J. A. Baptista Centro de Biologia Molecular, Instituto de Pesquisas Energéticas e Nucleares, IPEN/CNEN Avenida Prof. Lineu Prestes 2242, S?o Paulo, 05508-000, Brazil

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P. J. Spencer Centro de Biologia Molecular, Instituto de Pesquisas Energéticas e Nucleares, IPEN/CNEN Avenida Prof. Lineu Prestes 2242, S?o Paulo, 05508-000, Brazil

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J. E. Oliveira Centro de Biologia Molecular, Instituto de Pesquisas Energéticas e Nucleares, IPEN/CNEN Avenida Prof. Lineu Prestes 2242, S?o Paulo, 05508-000, Brazil

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M. S. Casare Centro de Biologia Molecular, Instituto de Pesquisas Energéticas e Nucleares, IPEN/CNEN Avenida Prof. Lineu Prestes 2242, S?o Paulo, 05508-000, Brazil

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N. Nascimento Centro de Biologia Molecular, Instituto de Pesquisas Energéticas e Nucleares, IPEN/CNEN Avenida Prof. Lineu Prestes 2242, S?o Paulo, 05508-000, Brazil

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Summary  

In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase from Bothrops jararacussu, and of ovalbumin before and after irradiation with 60Co g-rays. Isogenic mice were immunized with either native or irradiated proteins. The circulating antibodies were isotyped and titrated by ELISA. Results indicate that irradiated proteins were immunogenic and the antibodies elicited by them were able to recognize the native proteins in ELISA. Data also indicate that the irradiated protein induced higher titers of IgG2a and IgG2b, suggesting that Th1 cells were predominantly involved in the immune response. Structural modifications of the proteins were investigated by SDS-PAGE, mass spectrometry and size exclusion chromatography. According to our data, irradiation promoted structural modifications on both proteins, characterized by higher molecular weight forms (aggregates and oligomers). When analyzed by mass spectrometry, the irradiated bothropstoxin appeared in several oxidized forms. These results indicate that irradiation of toxic proteins can promote significant modifications in their structures, but still retain many of the original antigenic and immunological properties of native form.

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Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation
1968
Volumes
per Year
1
Issues
per Year
12
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)