The use of radiotracers in the research of animal venom has been scarce, although it allows an excellent approach to follow
the process of bioavailability, biodistribution and kinetics of toxins. The purpose of this study was to assess gyroxin action
mechanism, transport, compartments and action sites. This toxin is a thrombin-like and causes the barrel rotation syndrome.
The gyroxin was labeled with 125I and used as a tracer for the in vivo assay in mice. Blood samples and organs were collected at different time intervals,
weighed and analyzed in a gamma-counter. The data was related with tissues distribution of protease activated receptor (PAR).
Biodistribution assay allowed dividing the organs into three groups. The first one with the organs that followed the blood
kinetics, the second with the organs related to metabolisms and elimination, and the third with the organs in which the gyroxin
concentration increased during the observation period.