PACAP-27 radioimmunoassay: Description and application of a novel method

J. Németh; B. Jakab; R. Józsa; T. Hollósy; A. Tamás; A. Lubics; I. Lengvári; P. Kiss; Zs. Oberritter; B. Horváth; Z. Szilvássy; D. Reglődi

doi:10.1007/s10967-007-6862-3

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Journal of Radioanalytical and Nuclear Chemistry

Volume/Issue:: Volume 273: Issue 2

PACAP-27 radioimmunoassay: Description and application of a novel method

Authors:

J. Németh

J. Németh Neuropharmacology Research Group of the Hungarian Academy of Sciences Department of Pharmacology and Pharmacotherapy Pécs Hungary

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B. Jakab

B. Jakab Neuropharmacology Research Group of the Hungarian Academy of Sciences Department of Pharmacology and Pharmacotherapy Pécs Hungary

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R. Józsa

R. Józsa Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences Department of Anatomy Pécs Hungary

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T. Hollósy

T. Hollósy Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences Department of Anatomy Pécs Hungary

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A. Tamás

A. Tamás Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences Department of Anatomy Pécs Hungary

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A. Lubics

A. Lubics Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences Department of Anatomy Pécs Hungary

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I. Lengvári

I. Lengvári Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences Department of Anatomy Pécs Hungary

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P. Kiss

P. Kiss Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences Department of Anatomy Pécs Hungary

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Zs. Oberritter

Zs. Oberritter University of Pécs Department of Pediatrics Szigeti út 12 H-7624 Pécs Hungary

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B. Horváth

B. Horváth Neuropharmacology Research Group of the Hungarian Academy of Sciences Department of Pharmacology and Pharmacotherapy Pécs Hungary

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Z. Szilvássy

Z. Szilvássy University of Debrecen Department of Pharmacology and Pharmacotherapy Nagyerdei krt. 98 H-4019 Debrecen Hungary

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, and

D. Reglődi

D. Reglődi Neurohumoral Regulations Research Group of the Hungarian Academy of Sciences Department of Anatomy Pécs Hungary

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Pages:: 327–332

Online Publication Date:: 30 Jun 2007

Publication Date:: 01 Aug 2007

Article Category:: Research Article

DOI:: https://doi.org/10.1007/s10967-007-6862-3

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Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a widely distributed neuropeptide that has two molecular forms with 38 and 27 amino acid residues. The aim of the present study was to develop a new, highly specific PACAP-27 assay to investigate the quantitative distribution of PACAP-27 in the central nervous system of various vertebrate species applying the same technical and experimental conditions. Our results show that the antiserum used turned to be PACAP-27 specific. The average ID₅₀ value was 51.5±3.6 fmol/ml and the detection limit was 2 fmol/ml. PACAP-27 immunoreactivity was present in the examined brain areas, with highest concentration in the rat diencephalon and telencephalon. Swine and pigeon brain also contained significant amount of PACAP-27. Our results confirm the previously described data showing that PACAP-38 is the dominant form of PACAP in vertebrates, since PACAP-38 levels exceeded those of PACAP-27 in all examined brain areas. Furthermore, our study describes for the first time, the comparative quantitative distribution of PACAP-27 and-38 in the swine and pigeon brain.

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Journal of Radioanalytical and Nuclear Chemistry

Print ISSN:: 0236-5731

Online ISSN:: 1588-2780

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Journal of Radionalytical and Nuclear Chemistry
Language	English
Size	A4
Year of Foundation	1968
Volumes per Year	1
Issues per Year	12
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0236-5731 (Print)
ISSN	1588-2780 (Online)