U(VI) was quantitatively extracted from 1·10−3M HNO3 using 5·10−3M Cyanex 302 in xylene and was stripped from organic phase with 5M HCl. The optimum extraction conditions have been evaluated by studying parameters like acidity, effect of diluents, extractant concentration and period of equilibration. Based on this data, the separations of uranium from binary and complex metal mixtures and its recovery from uranmicrolite tailings (leachate) were successfully tested. Uranium can be determined with a relative standard deviation of 0.4%.