Journal of Radioanalytical and Nuclear Chemistry
Volume/Issue:
Volume 274: Issue 2
Study on the apoptosis and Bcl-2/Bax expression of human hepatoma SMMC-7721 cells after exposure to heavy-ion and X-ray irradiations
Authors:
Lanping Ye Lanzhou University School of Life Sciences Lanzhou 730000 P.R. China

Search for other papers by Lanping Ye in
Current site
Google Scholar
PubMed Close
,
Hua Xu Lanzhou University School of Life Sciences Lanzhou 730000 P.R. China

Search for other papers by Hua Xu in
Current site
Google Scholar
PubMed Close
,
Xiaodong Jin Chinese Academy of Sciences Institute of Modern Physics Lanzhou 730000 P.R. China

Search for other papers by Xiaodong Jin in
Current site
Google Scholar
PubMed Close
,
Qiang Li Chinese Academy of Sciences Institute of Modern Physics Lanzhou 730000 P.R. China

Search for other papers by Qiang Li in
Current site
Google Scholar
PubMed Close
, and
Lizhe An Lanzhou University School of Life Sciences Lanzhou 730000 P.R. China

Search for other papers by Lizhe An in
Current site
Google Scholar
PubMed Close
Pages:
429–434
Online Publication Date:
11 Jul 2007
Publication Date:
01 Nov 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1007/s10967-007-1132-y
Restricted access

Abstract  

This study is aimed at observing the apoptosis and Bcl-2/Bax gene expression of mammalian cells following heavy-ion and X-ray irradiations. Exponentially growing human hepatoma SMMC-7721 cells cultured in vitro were irradiated with a 12C ion beam of 50 MeV/u (corresponding to a LET value of 44.56 keV/μm) from Heavy Ion Research Facility in Lanzhou (HIRFL) at doses varying from 0 to 3 Gy. The X-ray irradiation (8 MV) was performed in the therapy unit of the General Hospital of the Lanzhou Military Area. Survival fractions of irradiated cells at various doses were measured by means of MTT assay. Apoptotic cells after irradiation were analyzed with fluorescence microscope and flow cytometer (FCM). Immuno-histological assay were applied to detect the expression of Bcl-2/Bax genes in the irradiated cells. The survival fraction of SMMC-7721 cells decreased gradually (vs. control p<0.05) with increasing the dose of the carbon ion beam more obviously than X-ray irradiation, and the carbon ion irradiation efficiently induced cell apoptosis and significantly promoted the expression of Bax gene while Bcl-2 gene expression was restrained. High-LET heavy ion beam would induce cell apoptosis effectively than low-LET X-ray, and the apoptosis rate is correlated with the transcription of Bcl-2/Bax and the ratio of Bcl-2/Bax in human hepatoma SMMC-7721 cells after irradiation to heavy ion beam.

  • Collapse
  • Expand
Cover Journal of Radioanalytical and Nuclear Chemistry
Journal of Radioanalytical and Nuclear Chemistry
Print ISSN:
0236-5731
Online ISSN:
1588-2780

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Radionalytical and Nuclear Chemistry
Language English
Size A4
Year of
Foundation		 1968
Volumes
per Year		 1
Issues
per Year		 12
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5731 (Print)
ISSN 1588-2780 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Sep 2024 5 0 0
Oct 2024 33 0 0
Nov 2024 8 0 0
Dec 2024 4 0 0
Jan 2025 8 0 0
Feb 2025 11 0 0
Mar 2025 11 0 0